| 刘海,张荣意,谭志琼,阮云泽.一株香蕉枯萎病拮抗菌的鉴定及香蕉组培袋装苗防病试验[J].中国南方果树,2011,40(3): |
| 一株香蕉枯萎病拮抗菌的鉴定及香蕉组培袋装苗防病试验 |
| Identification of a strain of antagonistic bacterium against Fusarium oxysporum f.sp.cubense and Control experiment of bag seedlings of tissue culture |
| 投稿时间：2011-03-14 修订日期：2011-03-13 |
| DOI： |
| 中文关键词: 香蕉枯萎病菌 枯草芽孢杆菌；拮抗；袋装苗 |
| 英文关键词:Fusarium oxysporum f.sp. cubense Bacillus subtilis antagonism Seedling bags |
| 基金项目:农业部公益性行业科研专项（201103004）；海南大学热带作物种质资源保护与开发利用教育部重点实验室开放基金 |
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| 中文摘要: |
| 采用平板对峙法从海南省土壤中分离到5株对香蕉枯萎病有较强拮抗作用的芽孢杆菌,经形态特征及16S rDNA序列将菌株S40鉴定为枯草芽孢杆菌Bacillus subtilis。菌株S40的拮抗试验表明，不同浓度的发酵滤液均可抑制病原菌菌丝生长和孢子的萌发和生长。组培袋装苗实验表明拮抗菌可明显推迟其发病和死亡时间,三种不同处理的相对防治效果分别为71.2%、83.3%和92.4%。 |
| 英文摘要: |
| Five strains of Bacillus subtilis antagonistic to banana wilt disease were isolated from rubber plantation soil by means of Flat Confrontation method.Strain of 40 were identified as Bacillus subtilis.The antagonistic tests of strain of 40 showed that different concentrations of fermentation filtrates could inhibit mycelia growth and spore germination Seedling bags of tissue culture experiments revealed that the antagonists could significantly delay its outbreak of disease and time of death. .It has been concluded that comparative prevention effect of three different treatments were 71.2%、83.3% and 92.4% respectively. |
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