| 代鹏.几种重要水果采后病害拮抗细菌的筛选与初步鉴定[J].中国南方果树,2011,40(6): |
| 几种重要水果采后病害拮抗细菌的筛选与初步鉴定 |
| The screening and research of antagonistic bacteria of postharvest |
| 投稿时间：2011-09-01 修订日期：2011-09-21 |
| DOI： |
| 中文关键词: 拮抗细菌、果蔬贮藏、鉴定 |
| 英文关键词:Antagonistic bacterium Fruits -storage Identification |
| 基金项目:中央级公益性科研院所科研业务专项（No.2011hzs1J021）,海南省自然科学基金（（No.808178）资助 |
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| 中文摘要: |
| 从山东、安徽、湖北等7地采集土样40份，分离得到903株非病原性细菌，通过平板对峙培养，筛选出对草莓灰霉（Botrytis cinerea）、柑橘绿霉（Penicilliium digitatum）等五种水果常见采后病害菌株均有明显抑菌活性的40个菌株。室内果蔬贮藏保鲜试验表明， TL-2、TL-6、TL-8菌株的菌含量达到1x107cfu/ml时，均表现出较好的贮藏保鲜效果。经形态和生理生化鉴定，表现最好的两株拮抗菌TL-2和TL-6菌株的分类地位为枯草芽孢杆菌(Bacillus subtitles)。 |
| 英文摘要: |
| 903 bacteria strains were isolated from 40 soil samples collected from Shandong，Anhui，Sichuan，Chongqing，Shanghai and Hubei, and 40 antagonists strains against the primary postharvest fruit diseases (Botrytis cinerea, Penicilliium digitatum, Penicilliium expansum, Alternaria kikuchiana Tabaka and Physalospora piricola) were screened through dual culture method. In the storage test, antagonists strains TL-2、TL-6 and TL-8 all showed good result for fruit fresh keeping when their content reach to 1×107cfu/ml. According to the morphological, physiological and biochemical features, the best TL-2 and TL-6 antagonistic bacteria were identified and classified as Bacillus subtilis. |
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