| 李玲娣,刘金香,田晓.蚜虫体内柑橘衰退病毒分子生物学检测研究进展[J].中国南方果树,2012,41(4): |
| 蚜虫体内柑橘衰退病毒分子生物学检测研究进展 |
| Advances in Research of Molecular Biological Detection of Citrus tristeza virus in Aphid |
| 投稿时间：2011-10-13 修订日期：2012-05-24 |
| DOI： |
| 中文关键词: 分子检测 柑橘衰退病毒 蚜虫 |
| 英文关键词:Molecular detection Citrus tristeza virus (CTV) Aphid |
| 基金项目:农业部公益性行业科研专项（200903004-06），教育部创新团队（IRT0976），西南大学基本科研业务费（XDJK2009C133）资助 |
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| 中文摘要: |
| 由柑橘衰退病毒（Citrus tristeza virus, CTV）引起的柑橘衰退病是具有经济重要性的病害，褐色橘蚜是CTV的最有效传播介体。应用分子生物学方法检测蚜虫体内CTV已被研究多年，本文重点阐述了定性检测和定量检测，介绍了反转录PCR(RT-PCR)、反转录巢式PCR (RT-nested-PCR)、以及实时RT-PCR (Real-time RT-PCR)的应用。同时，对这些技术应用于研究CTV蚜传机理和CTV-介体-寄主三者的互作关系方面做了简要展望。 |
| 英文摘要: |
| Tristeza caused by Citrus tristeza virus (CTV) is an economically important citrus disease which has caused great loss to the citrus industry and Toxoptera citricida（Kirkaldy）is the most efficient vector. Molecular methods for detection of CTV in aphid has been studied for years. In this paper the great attention is particularly focused on qualitative and quantitative detection, molecular biological detection techniques of RT-PCR, RT-nested PCR and real-time RT-PCR are introduced. Further research is needed to focuse on applying these molecular biological detection techniques on mechanism of aphids spreading the CTV and the relationship of aphids-CTV-citrus. |
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