| 陶书田,Khanizadeh Shahrokh,赵梅,张彪,张绍铃.梨果实中肉桂醇脱氢酶基因的克隆与序列分析[J].中国南方果树,2012,41(3): |
| 梨果实中肉桂醇脱氢酶基因的克隆与序列分析 |
| Cloning and Analyzing of Cinnamyl Alcohol Dehydrogenase Gene from Pear (Pyrus) Fruit |
| 投稿时间：2011-11-22 修订日期：2012-03-12 |
| DOI： |
| 中文关键词: 梨 肉桂醇脱氢酶 RT-PCR |
| 英文关键词:Pear (Pyrus) Cinnamyl alcohol dehydrogenase PT-PCR |
| 基金项目:国家自然科学基金项目（31000888）；现代农业产业技术体系建设专项资金（CARS-29）;江苏省博士后科研基金（1002018B） |
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| 中文摘要: |
| 肉桂醇脱氢酶( CAD ) 是木质素生物合成过程中的一个关键酶。本研究分别提取了‘砀山酥梨’及‘幸水’梨果肉总RNA，并通过RT-PCR、克隆和测序，成功获得了2个CAD的大小约为689bp的cDNA片段，并分别命名为PB-CAD（登录号：FJ478151）和PP-CAD（登录号：FJ478152）。在核苷酸水平上，这两个基因片段只有4个碱基的差异，编码相似性达99.6%的两个氨基酸序列，该序列由229个残基组成。通过蛋白质序列比较，发现梨果实CAD与其他植物中CAD氨基酸序列极为相似，与苹果Malus domestica （AAC06319）、梅Prunus mume （BAE48658）、枇杷Eriobotrya japonica （ABV44810）和葡萄Vitis vinifera （CAO21890）的相似性分别达到97.8%、95%、92.6%和83.4%。 |
| 英文摘要: |
| Cinnamyl alcohol dehydrogenase (CAD) is a key enzyme during lignin biosynthesizing. Two gene fragments coding for CAD designated as PB-CAD ( GenBank accession No.FJ478151) and PP-CAD ( GenBank accession No. FJ478152) were cloned and sequenced using RT-PCR from ‘Dangshansuli’ and ‘Kousui’ pear, respectively. Both of the two obtained CAD gene fragments are 689 bp, and they have only 4 different bases in nucleotide sequence, which was comprised of 229 residues, and the two identical amino acid sequences have a 99.6% identity to each other. Using sequence alignment, we found the pear amino acid sequence of the two CAD was 97.8% identical to CAD from Malus domestica （AAC06319）, 95% identical to CAD from Prunus mume （BAE48658）, 92.6% identical to CAD from Eriobotrya japonica （ABV44810） and 83.4% identical to CAD from Vitis vinifera （CAO21890）, respectively. |
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