| 建德锋.越橘叶片组培快繁技术研究[J].中国南方果树,2012,41(5): |
| 越橘叶片组培快繁技术研究 |
| Leaf Culture of Vaccinium vitis-idaea in Vitro |
| 投稿时间：2012-05-18 修订日期：2012-05-18 |
| DOI： |
| 中文关键词: 越橘 叶片 培养基 植物激素 |
| 英文关键词:Vaccinium vitis-idaea L leaf medium plant hormones |
| 基金项目: |
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| 摘要点击次数: 2358 |
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| 中文摘要: |
| 以越橘的叶片为材料进行组培技术研究，结果发现把经过消毒灭菌的叶片切块后接种到1/4Anderson 2,4-D1mg/L TDZ0.1 mg/L培养基上，愈伤组织诱导效果最好，然后把分化出的愈伤组织切割转入1/4Anderson IBA0.1mg/L TDZ1mg/L 2-IP0.5mg/L培养基上进行芽的诱导效果最佳，最好将芽转入1/4Anderson IBA2mg/L或1/4Anderson NAA0.1mg/L培养基上进行生根培养，生根效果较好。 |
| 英文摘要: |
| Taking the leaves of Vaccinium vitis-idaea as materials to do the study of culture in vitro, the results showed that the results of callus induction were the best in the medium of 1/4Anderson 2,4-D1mg/L TDZ0.1 mg/L, in which leaf were cut and seeded after disinfection and sterilization. The callus were cut and transferred to the medium of 1/4Anderson IBA0.1mg/L TDZ1mg/L 2-IP0.5mg/L, and the effects of bud induction were the best. At last, the buds were transferred to the medium of 1/4Anderson IBA2mg/L or the medium of 1/4Anderson NAA0.1mg/L to do rooting culture, and the effects were better. |
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