| 左佩佩,李敏敏,胡军华.岩白菜内酯对柑桔病原真菌抑制作用研究[J].中国南方果树,2012,41(4): |
| 岩白菜内酯对柑桔病原真菌抑制作用研究 |
| Inhibition effect of bergenin against citrus pathogens |
| 投稿时间：2012-05-24 修订日期：2012-05-24 |
| DOI： |
| 中文关键词: 岩白菜内酯 柑桔病原真菌 抑菌活性 |
| 英文关键词:bergenin citrus antifungal activity |
| 基金项目: |
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| 摘要点击次数: 2281 |
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| 中文摘要: |
| 本试验采用生长速率法测定了岩白菜内酯对柑桔7种病原菌的抑制活性，通过显微观察和生长量测定研究了岩白菜内酯抑制柑橘炭疽病菌的机理并进行了果实贮藏实验。结果表明，岩白菜内酯能够抑制7种病原菌的生长，随着浓度的增加，抑制率增大，呈现正相关变化，其中对柑桔青霉菌的抑制效果最好，EC50为0.3996 mg/L。岩白菜内酯处理的柑桔炭疽菌菌丝隔膜大部分消失，仅极少数存在隔膜，菌丝扭曲畸形，两侧菌丝壁粗糙、不对称。分生孢子油胞消失。岩白菜内酯浓度在20礸/mL时对柑桔炭疽菌菌丝生长有着明显的抑制作用(见表2图3)。第3d时对照菌丝干重为0.4415g，而岩白菜内酯处理的菌丝干重为0.1612g，抑制率达到61.74%。随后几天岩白菜内酯的抑制作用减弱，抑制效率明显降低，在第6d抑制率仅为2.31%。在果实贮藏试验中，岩白菜提取物的浓度越高对柑桔贮藏病害的抑制效果越好。0.006g/mL岩白菜粗提物对青霉病的防治效果最佳。表明岩白菜内酯在柑桔病原真菌室内毒力测定及在果实贮藏试验中都表现出很好的抑菌作用，因此对岩白菜内酯抑菌机理和资源化利用的研究显得尤为重要，这些研究将为岩白菜内酯类生物农药的开发提供依据。 |
| 英文摘要: |
| Antifungal activity of bergenin against Penicillium italicum、Penicillium digitatum、Colletotrichum 、Phytophthora parasitica、Diaporthe medusaea、Alternaria citri and Guignardia citricarpa was tested in laboratory by using growth rate methods.，and storage experiment Of Bergenin。Bergenin on these 6 kinds of fungal hyphae growth has a very good inhibitory effect, with the increase of concentration, the inhibition rate increase, is positively related to changes.The citrus Penicillium had the best inhibition effect, at a concentration of 40 g/mL, the inhibition rate was 77.14%.In fruit storage test, the higher concentration of Bergenia extract citrus storage diseases inhibition effect to be better.0.006g / mL Bergenia crude extracts on Penicillium disease effect best.Show bergenin in Citrus pathogenic fungi indoor toxicity determination and in fruit storage tests have shown a very good antibacterial effect, so the bergenin antibacterial mechanism and resource utilization study appears to be particularly important, these studies will provide bergenin class provides the basis for the development of biological pesticide. |
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