| 易龙,马冠华.柑橘溃疡病拮抗芽孢菌株的筛选及其控病作用[J].中国南方果树,2012,41(4): |
| 柑橘溃疡病拮抗芽孢菌株的筛选及其控病作用 |
| Screening of Antagonistic Spore Bacteria for Biocontrol against Xanthomonas axonopdis pv. citri |
| 投稿时间：2012-06-24 修订日期：2012-06-24 |
| DOI： |
| 中文关键词: 柑橘溃疡病 枯草芽孢杆菌 生物防治 |
| 英文关键词:Citrus bacterial canker Bacillus subtilis Biocontrol |
| 基金项目:公益性行业（农业）科研专项经费（201003067-02-6; 201003067-02-7），江西省科技支撑计划项目（2010BNB01402） |
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| 中文摘要: |
| 从柑橘根际土壤中获得192个细菌分离物，通过平板拮抗测试，筛选出对柑橘溃疡病菌（Xanthomonas axonopdis pv. citri）有较强拮抗作用的6个芽孢菌株，抑菌圈直径达12.0～25.0mm，其中菌株G32对柑橘溃疡病菌抑制效果最好，稳定性高；温室控病试验表明，菌株G32的控病效果最佳，提前喷施拮抗菌其预防效果达60.7%，接种溃疡病菌后喷施的治疗效果为53.2%；根据其形态特征、培养性状、生理生化特性及16S rDNA序列比对分析，将G32鉴定为枯草芽孢杆菌Bacillus subtilis。 |
| 英文摘要: |
| 192 isolates were obtained from rhizosphere soil of citrus fields collected from Ganzhou in Jiangxi. It was found that 6 potential biocontrol spore bacteria showed good antagonistic effect on Xanthomonas axonopdis pv. citri, the pathogen of citrus bacterial canker, and the inhibition zone diameters ranged from 12.0-25.0 mm by pairing culture on NA plate, the bacterial strain G32 has high activity to the pathogen. It was found that the control efficacy of strain G32 reaching to 60.7% by pre-spraying antagonistic bacteria, and 53.2% by spraying antagonistic bacteria after pathogen inoculation in a greenhouse experiment. According to the characteristics of morphological, cultural, physiological and biochemical, as well as 16S rDNA sequence, the strain G32 was identified as Bacillus subtilis. |
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