| 黄文静,金俊军,高凡,杨光柱,郑丽萍,马钧.火龙果杂交实生苗快速繁育技术研究[J].中国南方果树,2013,42(1): |
| 火龙果杂交实生苗快速繁育技术研究 |
| The breeding technology research of Pitaya hybrid seedling |
| 投稿时间：2012-08-20 修订日期：2012-11-27 |
| DOI： |
| 中文关键词: 火龙果 杂交实生苗 繁育 |
| 英文关键词:Red Pitaya Hybrid seedlings Rapid propagation |
| 基金项目: |
|
| 摘要点击次数: 10139 |
| 全文下载次数: 4853 |
| 中文摘要: |
| 以杂交得到的红皮红肉型火龙果自然成熟果实的种子为外植体，进行改良的组织快繁实验，筛选适宜的培养基、激素浓度配比，并对组培苗进行了移栽。结果显示，萌芽诱导培养1/2MS NAA0.3 mg/L。实生苗增殖培养MS 6-BA5.0 mg/L NAA0.3 mg/L，增殖系数可达，且芽生长势强，质量好。子叶二次萌芽培养1/2MS 6-BA1.5 mg/L，生根培养1/2MS NAA0.3mg/L IBA0.3 mg/L，生根率达95%。炼苗后7天的幼苗移栽至处理好的苗床上，20天后成活率达96%以上。 |
| 英文摘要: |
| The stem segments with natural ripe fruit seeds of the red Pitaya hybrid offspring were used as experimental explants to study the different disinfection methods and the effects of hormone on adventitious bud occurrence and multiplication. The results indicated that: the medium of 1/2MS NAA0.3 mg/L was best for bud infection. The optimal multiplication medium in subculture was MS 6-BA5.0 mg/L NAA0.3 mg/L, the multiplication rate was above 4.9, and the new bud had strong growth potential. The medium of 1/2MS 6-BA1.5 mg/L was best for the second bud infection of cotyledon. 1/2MS NAA0.3mg/L IBA0.3 mg/L was best medium for rooting, with a rooting of 95%. After acclimatization for 7 days, the survival rate was up to 96% on 20 days after transplanted into sterilized medium. |
| 查看/发表评论 下载PDF阅读器 |
| 关闭 |
