| 易龙.柑橘裂皮类病毒江西分离物（CEVd-RJ）全长cDNA序列分析[J].中国南方果树,2012,41(6): |
| 柑橘裂皮类病毒江西分离物（CEVd-RJ）全长cDNA序列分析 |
| Analyzing a Full Length cDNA of Citrus Exocortis Viroid CEVd-RJ Isolate from Jiangxi |
| 投稿时间：2012-08-22 修订日期：2012-08-30 |
| DOI： |
| 中文关键词: 柑橘裂皮类病毒 江西 全长cDNA 序列分析 |
| 英文关键词:Citrus exocortis viroid Jiangxi Full length cDNA Sequence analysis |
| 基金项目:江西省青年科学家培养计划（2010DQ02300），江西省科技支撑计划项目（2010BNB01402） |
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| 中文摘要: |
| 运用RT-PCR技术对柑橘裂皮类病毒江西分离物CEVd-RJ的全长cDNA序列进行扩增、克隆，并对其片段进行序列测定。将获得的371bp的全长cDNA序列与GenBank登录的柑橘裂皮类病毒相应序列进行比对分析，结果发现江西分离物CEVd-RJ与国内柑橘裂皮类病毒湖北分离物CEVd-HB的同源性为99.7%，与广东分离物CEVd-YC的同源性为90.8%，同国外9个分离物间的同源性在89.0%~98.6%之间。序列差异分析发现柑橘裂皮类病毒江西分离物CEVd-RJ的全长cDNA序列比广东分离物CEVd-YC少一个碱基，有36个位点碱基发生了变化，与湖北分离物CEVd-HB存在一个碱基位点的差异。 |
| 英文摘要: |
| The Citrus exocortis viroid isolate CEVd-RJ from Jiangxi province were detected by RT-PCR, and the full length cDNA was sequenced and compared with those corresponding sequences of Citrus exocortis viroid isolates from GenBank by BioEdit and MEGA softwares. The results showed that CEVd-RJ isolate has highly 99.7% homologies with CEVd-HB which originated from Hubei province, and 90.8% lowly homologies with CEVd-YC isolate from Guangdong province. The cDNA sequence of CEVd-RJ isolate has identity ranging from 89.0% to 98.6% with nine Citrus exocortis viroid isolates of foreign countries. The sequence of cDNA of CEVd-RJ isolate was compared with those of CEVd-HB and CEVd-YC isolates, the results showed that the full length cDNA of CEVd-RJ isolate has one base less than that of CEVd-YC isolate, with 36 variable sites, and there was one base site have changed compared with CEVd-HB. |
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