| 郑伟尉,吕锡山,邵泱峰,慎家辉,邵晓岚,徐凯,臧运祥.兔眼蓝莓‘园蓝’离体培养增殖技术研究[J].中国南方果树,2013,42(1): |
| 兔眼蓝莓‘园蓝’离体培养增殖技术研究 |
| Study on in vitro Multiplication of Rabbit Eye Blueberry Garden Blue |
| 投稿时间：2012-11-05 修订日期：2012-11-05 |
| DOI： |
| 中文关键词: 兔眼蓝莓 组织培养 增殖 |
| 英文关键词:rabbit eye blueberry tissue culture multiplication |
| 基金项目: |
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| 中文摘要: |
| 本试验以兔眼蓝莓主栽品种‘园蓝’为试材，研究其离体培养增殖技术。基本培养基为WPM培养基，添加蔗糖20g/L，琼脂10g/L，pH为5.2。实验总体分为两个过程：第一步，在基本培养基中加入ZT (2mg/L, 4mg/L, 6mg/L)或2ip (2mg/L, 5mg/L, 10mg/L)，共6个处理，培养60d后，观察记录蓝莓腋芽萌发情况，WPM ZT 4mg/L更有利于腋芽萌发，各处理中腋芽萌发后生长速度都很慢；第二步，在基本培养基中加入4mg/L的ZT及IAA（0.1mg/L, 0.2mg/L）或IBA（0.1mg/L, 0.2mg/L），共4个处理，培养60d后进行数据统计，发现最佳处理为WPM ZT 4mg/L IBA 0.2 mg/L，并且芽子长势较好。因此，兔眼蓝莓‘园蓝’腋芽诱导和生长的最佳培养基为WPM ZT 4mg/L IBA 0.2 mg/L。 |
| 英文摘要: |
| This work studied the in vitro multiplication of rabbit eye blueberry Garden Blue. The basic medium used is WPM plus sucrose 20g/L, agar 10g/L, with pH of 5.2. Two experiments were performed as follows. One was carried out as WPM plus ZT (2mg/L, 4mg/L, 6mg/L) or 2ip(2mg/L, 5mg/L, 10mg/L). Data were collected after 60 days. The results indicate that the best treatment for shoot formation from axillary buds is WPM plus ZT 4mg/L, shoots formed from all treatments grow very slowly. Another was performed as WPM 4mg/L of ZT supplemented with IAA (0.1mg/L, 0.2mg /L) or IBA (0.1mg/L, 0.2mg/L). 60 days later, we found that the best treatment for shoot formation is WPM ZT 4mg/L IBA 0.2 mg/L, and those shoots grow well. As a result, the best medium for shoot formation and growth from axillary buds of Garden Blue is WPM ZT 4mg/L IBA 0.2 mg/L. |
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