| 李晓娇,岳建苏,陈飞,张云飞,冉春.柑橘全爪螨不同品系谷胱甘肽S-转移酶基因表达差异分析[J].中国南方果树,2013,42(3): |
| 柑橘全爪螨不同品系谷胱甘肽S-转移酶基因表达差异分析 |
| Difference analysis of glutathione S-transferase gene between different strains in the citrus red mite, Panonychus citri (Acari: Tetranychidae) |
| 投稿时间：2012-11-13 修订日期：2013-01-06 |
| DOI： |
| 中文关键词: 柑橘全爪螨 噻螨酮 抗性 谷胱甘肽S-转移酶基因 差异分析 |
| 英文关键词:Panonychus citri Hexythiazox Resistance glutathione S-transferase gene Difference analysis |
| 基金项目: |
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| 中文摘要: |
| 为了明确谷胱甘肽S-转移酶（glutathion S-transferases, GSTs）基因与柑橘全爪螨抗性的关系，通过BLAST搜索，对柑橘全爪螨转录组数据库的GST基因进行鉴定，应用MEGA4.0.1软件对GST基因进行同源与进化分析，进一步采用RPKM法对柑橘全爪螨敏感品系和噻螨酮抗性品系GST基因进行表达差异分析。从柑橘全爪螨转录组中获得了30条GST基因，其中11条基因属于Delta家族，10条属于Mu家族，2条属于Omega家族，6条属于Kappa家族，1条属于Zeta家族。基因表达差异分析发现，抗性与敏感品系当中有25条GST基因表达量存在差异，抗性品系中有9条GST基因发生了上调，16条发生了下调，其中上调倍数最高的是GSTd6[log2 Ratio(RS/SS)= 1.0505]，由此推断，GST基因的上调可能不是柑橘全爪螨对噻螨酮产生抗性的重要原因。 |
| 英文摘要: |
| In order to clarify the relationship between glutathione S-transferase(GST) gene and resistance of Panonychus citri, GST genes information were identified from the P. citri transcriptome database by BLAST searching, and phylogenetic tree was constructed by MEGA4.0.1. Gene expression profiles of GST genes between the hexythiaz-resistant straint and susceptible strain were further compared using RPKM (Reads Per kb per Million reads) method. 30 GST genes were found in transcripts of P. citri, of which, 11, 10, 2, 6 and 1 GST genes belong to the Delta, Mu, Omega, Kappa family, and Zeta family, respectively. Gene expression difference analysis results indicated that 9 GST genes were up-regulated and 16 GST genes were down-regulated in the resistant straint, of which, GSTd6[log2 Ratio(RS/SS)= 1.0505]was the top up- regulated GST gene. The above results indicated that up-regulation of GST genes may not play an important role in hexythiazox resistance of P. citri. |
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