| 谢志亮.早食李基因组DNA的提取方法改进与试验[J].中国南方果树,2013,42(2): |
| 早食李基因组DNA的提取方法改进与试验 |
| The Improvement and Experiment on Total DNA Extraction of “Zaoshi” Plum |
| 投稿时间：2012-11-26 修订日期：2013-01-01 |
| DOI： |
| 中文关键词: 早食李 DNA提取方法 改进 |
| 英文关键词:“zaoshi” plum DNA extraction method Improvement |
| 基金项目:公益性行业（农业）科研专项（No.201003058）资助 |
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| 摘要点击次数: 2422 |
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| 中文摘要: |
| 以改进的CTAB法对三华李品种群中的早食李叶片基因组DNA的提取进行了试验。结果表明，改进的CTAB法适于高质量三华李品种群基因组DNA的提取。提取的早食李基因组DNA完整性较好，无降解，OD260/OD280的比值在1.9-2.0之间，多糖类杂质去除较为干净。经SRAP检验，条带清晰，多态性良好，说明改良CTAB法提取的基因组DNA质量较好，适于三华李高质量基因组DNA的提取。不同时期的不同成熟度的叶片都可以提取到基因组DNA，但以各期的嫩叶（梢）产量最高，效率最好。 |
| 英文摘要: |
| The DNA extraction experiment of‘zaoshi’plum was carried out by the improved CTAB DNA extraction method. The result indicated that the improved CTAB DNA extraction method was suitable for high quality DNA extraction of‘sanhua’plum cultivar group. The extracted‘zaoshi’plum DNA was integrality and without degradation, the OD260/OD280 radio was between 1.9-2.0, and the polysaccharide was dislodged clearly. The SRAP test produced clear polymorphic patterns indicated that the improved CTAB DNA extraction method was suitable for high quality DNA extraction of‘sanhua’plum cultivar group.. The leaf of different period and maturation were also suitable for DNA extraction, but tender leaf（shoots）were the best. |
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