| 赖传雅,李朝生,韦迎春,刘卫民,方月兰.龙眼叶枯病菌疏毛拟盘多毛孢生物学特性研究[J].中国南方果树,2013,42(4): |
| 龙眼叶枯病菌疏毛拟盘多毛孢生物学特性研究 |
| Biological properties of Pestalotiopsis pauciseta on longan leaf blight |
| 投稿时间：2013-06-12 修订日期：2013-06-27 |
| DOI： |
| 中文关键词: 龙眼叶枯病 疏毛拟盘多毛孢 生物学特性 营养条件 |
| 英文关键词:Longan leaf blight Pestalotiopsis pauciseta biological properties nutrition conditions |
| 基金项目:广西区发展计划委员会投字[1998年412号]项目 |
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| 中文摘要: |
| 龙眼叶枯病菌疏毛拟盘多毛孢生长温度10～30℃，最适生长温度20～25℃；在14～26℃下培养时，RH100%和 pH 6.3～7.2产孢量最大；分生孢子萌发率则以在25℃和RH+水滴及在24～26℃和pH 6～7时较高。黑暗处理促进菌丝生长，日光灯照射有利于分生孢子形成。分生孢子致死温度57℃10min。该菌能利用多种单糖、双糖和醇类作碳源，葡萄糖和蔗糖增进菌丝生长和孢子形成；能很好利用供试氮源L-白氨酸、L-胱氨酸和L-天冬氨酸等。L-组氨酸作为氮源时病菌产孢量最大，而氯化氨作为氮源时病菌不产孢。 |
| 英文摘要: |
| The causal agent responsible for the leaf blight of longan, Pestalotiopsis pauciseta Y.X.Chen, could grow at temperature between 10℃ and 30℃, with the optimal growth at 20-25℃; the best conidium for sporulation was at 14-26℃ with RH100% and pH 6.3-7.2 in artificial media; highest condition germination rate was obtained when the temperature was 25℃ with RH at 100% and water drop, or in water when the temperature was 24-26℃ and pH was 6-7. Treatment of darkness had significant effect on the mycelium growth .The treatment of fluorescent light could cause large scale production on conidium. The lethal temperature for the conidium was 57℃ 10 minutes. When cultured in artificial media, the pathogene could utilize monosacharides, disacharides as well as alcohols as carbon sources and L-leucine, L-cystine, L-aspartic acid et al. as nitrogen sources. The fungus could produce a large number of conidia when L-histidine was the only nitrogen source or did not sporulate when Ammonium chloride was the only nitrogen source. |
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