| 刘晓生,郑道序,周春娟,马瑞君,詹潮安,杨海东,杨培奎,庄东红.潮汕余甘子种质资源遗传多样性与亲缘关系的ISSR分析[J].中国南方果树,2014,43(1): |
| 潮汕余甘子种质资源遗传多样性与亲缘关系的ISSR分析 |
| Genetic Diversity and Relationship of Phyllanthus emblica L. Germplasm in Chao-Shan area Revealed by ISSR |
| 投稿时间：2013-09-06 修订日期：2013-10-10 |
| DOI： |
| 中文关键词: 余甘子 ISSR 遗传多样性 |
| 英文关键词:Phyllanthus emblica L Germplasm ISSR Genetic diversity |
| 基金项目:广东省科学技术厅科技基础条件建设项目 |
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| 中文摘要: |
| 为了分析潮汕地区所收集的17份余甘子品种（系）的遗传多样性，本研究采用ISSR分子标记技术进行研究。利用7条多态性引物共扩增出70条可重复条带，多态性条带有56条，多态位点百分率（PPB）为80%，分析揭示供试品种（系）的观察等位基因数、有效等位基因数、Nei’s遗传多样性指数和Shannon's信息指数分别为1.8000、1.4700、0.2703、0.4047；各品种（系）之间的遗传相似系数（GS）范围在0.5714-0.9间，平均遗传相似性系数为0.6943。结果表明试验的供试材料的遗传多样性水平较高，亲缘关系鉴定结果与根据样品来源、农艺性状及形态特征划分类别的结果基本一致。 |
| 英文摘要: |
| In order to analysis the genetic diversity of 17 Phyllanthus emblica L Germplasm varieties(strain) collected in east Guangdong,the ISSR markers were used to analysis the genetic diversity of 17 Phyllanthus emblica L Germplasm varieties(strain) . A total of 70 DNA bands were amplified, among which 56 (80%) were polymorphic. he the observed number of alleles (Na ), effective number of alleles (Ne ), Nei’s gene diversity (H) and Shannon’s information index (I ) of accessions were1.8000，1.4700，0.2703 and 0.4047, respectively. The genetic similarity computed by NTSYSpc 2.10e amony 12 varieties ranged from0.5714 to 0.9. The rerult showed that there was the relatively highgenotype diversity. The result of relationship among the cultivars with ISSR analysy were accords basically with that agronomic characters. |
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