周求根,李诚,蒋军喜.贡梨果实黑斑病病原菌鉴定及室内防治药剂筛选[J].中国南方果树,2014,43(3): |
贡梨果实黑斑病病原菌鉴定及室内防治药剂筛选 |
Identification of the pathogenic fungus of pear black spot and indoor screening of fungicides |
投稿时间:2013-10-11 修订日期:2014-04-05 |
DOI: |
中文关键词: 梨黑斑病 Alternaria alternate 毒力测定 EC50 |
英文关键词:pear black spot Alternaria alternate toxicity test EC50 |
基金项目:江西省教育厅科学技术研究项目(GJJ13269) |
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中文摘要: |
通过病原菌形态特征、致病性测定、rDNA-ITS序列和EF1-α基因序列分析,确定贡梨黑斑病的病原菌为链格孢菌(Alternaria alternate)。在此基础上,采用菌丝生长速率法测定8种杀菌剂对该病原菌的毒力大小,结果表明,苯醚甲环唑、异菌脲、肟菌?戊唑醇和苯甲?丙环唑的抑菌效果很好,EC50值分别为0.4904μg/mL、0.5091μg/mL、0.5718μg/mL和0.9138μg/mL。 |
英文摘要: |
The pathogen causing black spot on pear was identified as Alternaria alternate by morphological characteristics, pathogenicity test, and sequence analysis of rDNA-ITS and EF1-α. Indoor toxicity of eight fungicides were tested by using mycelium growth rate method. The results showed that four fungicides of Difenoconazole, Rovral, Trifloxystrobin?Tebuconazole and Difenoconazole?propiconazole had high inhibitory effects on Alternaria alternate, and the EC50 value was 0.4904μg/mL, 0.5091μg/mL, 0.5718μg/mL and 0.9138μg/mL, respectively. |
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