| 汪国云,周剑,沈禹彤,贾慧敏,高中山.应用多重荧光SSR标记组合鉴定杨梅果实的品种来源[J].中国南方果树,2014,43(3): |
| 应用多重荧光SSR标记组合鉴定杨梅果实的品种来源 |
| Using multiple fluorescent SSR makers to identify the cultivar sources of Chinese bayberry fruits |
| 投稿时间：2014-03-06 修订日期：2014-03-27 |
| DOI： |
| 中文关键词: 杨梅，多重PCR，果实品种鉴别，荧光SSR |
| 英文关键词:Chinese bayberry fruits Multiplex PCR Cultivar Identity Fluorescent-labeled SSR |
| 基金项目: |
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| 摘要点击次数: 2259 |
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| 中文摘要: |
| 为了快速有效的鉴别杨梅果实的品种来源，本研究采用从杨梅果实中提取DNA的方法，所用10个SSR引物添加不同颜色的荧光标记，建立四组多重PCR扩增体系，在高通量ABI3130遗传分析仪准确分析片段大小，参照已有的杨梅品种指纹数据库可以快速鉴定果实的品种属性。本文所介绍的方法可以在果实品质量检测以及优良品系选择中应用。 |
| 英文摘要: |
| To identify the different cultivar of Chinese bayberry fruits fast and efficiently, we extracted DNA from Chinese bayberry fruits, made use of 10 different color fluorescent-labeled SSR primers to establish suitable amplification conditions of 4 multiplex PCR for ABI 3130 high-throughput genetic analyzer. We provided a rapid and method for identifying the cultivar source by comparing with the established fingerprint profiles of major cultivated Chinese bayberries. This method can be used in inspection of fruit quality and identification of new line of Chinese bayberry. |
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