方智振,叶新福,周丹蓉,姜翠翠,廖汝玉,潘少霖.宁岗芙蓉李与福建芙蓉李的ISSR分析[J].中国南方果树,2014,43(5): |
宁岗芙蓉李与福建芙蓉李的ISSR分析 |
ISSR Analysis of Furong plums from Ninggang and Fujian |
投稿时间:2014-03-24 修订日期:2014-03-24 |
DOI: |
中文关键词: 芙蓉李 ISSR 遗传差异 |
英文关键词:Furong plum ISSR genetic difference |
基金项目:福建省科技重大专项专题、农业部公益类行业科研专项和福建省财政专项 |
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中文摘要: |
采用ISSR分子标记技术对宁岗芙蓉李与福建芙蓉李的遗传差异进行分析,从32条引物中筛选出10条引物对4个芙蓉李材料的DNA序列进行PCR扩增,共得到62个条带,多态性条带17个;宁岗芙蓉李与福建芙蓉李的ISSR指纹图谱差异显著,来自于不同地方的福建芙蓉李的ISSR指纹图谱之间无显著差异。引物UBC813、UBC818、BC825、UBC840、UBC844和UBC856均可以将宁岗芙蓉李与福建芙蓉李区分开。 |
英文摘要: |
Genetic differences between Furong plums from Ninggang and Fujian were detected by ISSR molecular markers. 10 primers were selected out of 32 primers that had been tested previously. 62 DNA bands were amplified, of which 17 bands were polymorphic. Significant differences were detected between ISSR fingerprintings of Furong plums from Ninggang and Fujian, no significant difference among Furong plums from different places in Fujian. Furong plums from Ninggang and Fujian could be distinguished by ISSR marker UBC813, UBC818, UBC825, UBC840, UBC844 and UBC856. |
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