| 马勉娣,黄治钰,张秀英,杨明挚.酿酒葡萄‘赤霞珠’叶内生真菌多样性研究[J].中国南方果树,2014,43(3): |
| 酿酒葡萄‘赤霞珠’叶内生真菌多样性研究 |
| Diversity of endophytic fungi in Wine Grape Cabernet Sauvignon leaves |
| 投稿时间：2014-04-15 修订日期：2014-04-24 |
| DOI： |
| 中文关键词: 酿酒葡萄赤霞珠、内生真菌，多样性，ITS序列分析 |
| 英文关键词:Cabernet Sauvignon wine grape leaves, endogenous fungi, community structure, diversity, ITS sequence analysis |
| 基金项目:云南葡萄属野生资源调查、保护及酿酒葡萄新资源发掘 |
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| 中文摘要: |
| 为探讨酿酒葡萄‘赤霞珠’叶片内生真菌群落结构和分布特征,本文采用贴片法对采自4个不同生境的 ‘赤霞珠’叶片可培养内生真菌进行分离，并通过分子生物学方法对分离的内生真菌进行鉴定。结果从采自四个生境的600个‘赤霞珠’叶片中共分离到内生真菌583株，归属于42个种22个属，其中链格孢属和黑孢属为优势属。德钦和石河子两地内生真菌分离率比较高, 大于1。而丘北的内生真菌多样性指数较其他生境高,为2.20。三种叶片中嫩叶的内生真菌的分离率和多样性指数明显低于成熟叶和老叶的。对不同生境‘赤霞珠’叶片的内生真菌的多样性指数及相似性分析结果表明，酿酒葡萄‘赤霞珠’叶片内生真菌菌群丰富存在一定的宿主专一性, 但多样性偏低。 |
| 英文摘要: |
| In order to study the endophytic fungi community structure and distribution in leaves of wine grape \"cabernet sauvignon\", Folia endophytic fungi from four different habitats of wine grape (Cabernet Sauvignon) were isolated and identified by molecular biological method. Results indicated that in total 583 strains of cultivable endophytic fungi were isolated from 600 pieces of grape leaf tissues, and all these fungi strains were identified into 42 species, 22 genus. Alternaria and Nigrospora are dominant genera. Isolation rate of folia endophytic fungi from Deqin county in Yunnan province and Shihezi in Xinjiang province was higher than other samples from Penglai Shandong province and Qiubei, Yunnan province, respectively. Diversity index of folia endophytic fungi from Qiubei, Yunnan province was up to 2.20 and higher than other wine producing areas. The isolation rate and the diversity indexes of folia endophytic fungi from younger leaves of folia endophytic fungi was lower significantly than that of the mature leaves and older leaves. According to the diversity indexes and similarity analysis results, we come to a conclusion that the wine grape ‘Cabernet Sauvignon’ leaves has a relatively low level of diversity, but some host specificity of endophytic fungi. |
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