| 胡振阳,谭志琼,徐刚,张荣意.香蕉枯萎病拮抗内生细菌的筛选及鉴定[J].中国南方果树,2014,43(5): |
| 香蕉枯萎病拮抗内生细菌的筛选及鉴定 |
| Screening and identification of antagonistic endophytic bacteria against banana fusarium wilt |
| 投稿时间：2014-05-06 修订日期：2014-05-22 |
| DOI： |
| 中文关键词: 内生细菌，香蕉枯萎病，拮抗菌，分离鉴定 |
| 英文关键词:Endophytic bacteria, Banana Fusarium wilt, Antagonistic bacteria, Isolation and identification |
| 基金项目:海南大学热带作物种质资源保护与开发利用教育部重点实验室开放基金；海南大学“211工程”建设经费资助项目。 |
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| 中文摘要: |
| 对健康香蕉植株体内的内生细菌进行了分离纯化，得到63株内生细菌菌株。采用平板对峙法筛选出2株对香蕉枯萎病具有强烈拮抗作用的菌株。这两株菌均能够显著抑制FOC菌丝生长, 抑菌率均在55%左右。通过生理生化、形态特征及16S rDNA序列同源性比较，鉴定均为枯草芽孢杆菌（Bacillus subtilis）。 |
| 英文摘要: |
| 63 endophytic bacterial strains were isolated and purified from the healthy banana plants, in which 2 antagonistic bacterial strains among them screened with slab confrontation method, had fairly strong inhibition effect to Bananas Fusarium wilt.The two strains obviously inhibited the mycelial growth of FOC,with an inhibitory rate of about 55%. They were identified as Bacillus subtilis based on the morphological, physiological and biochemical properties, and the 16S rDNA sequence analysis. |
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