| 宁进,张秀英,程玉,黄治钰,杨明挚.真菌诱导子对毛葡萄愈伤组织细胞次生代谢的影响[J].中国南方果树,2015,44(2): |
| 真菌诱导子对毛葡萄愈伤组织细胞次生代谢的影响 |
| Effects of Fungal Elicitors on Metabolism in Callus of Vitis heyneana |
| 投稿时间：2014-06-18 修订日期：2014-07-21 |
| DOI： |
| 中文关键词: 毛葡萄 愈伤组织 真菌诱导子 次生代谢 |
| 英文关键词:Vitis heyneana Callus Fungal elicitors Secondary metabolites |
| 基金项目:国家自然科学基金项目（面上项目，重点项目，重大项目） |
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| 中文摘要: |
| 以毛葡萄（Vitis heyneana Roemer & Schultes）愈伤组织为试验材料，研究了不同种类真菌水溶性诱导子及其不同浓度、不同添加时间和作用时间对毛葡萄愈伤组织细胞次生代谢产物的影响。结果表明：真菌诱导子的加入提高了毛葡萄细胞中总黄酮、总酚的含量及过氧化物酶（POD）、苯丙氨酸解氨酶（PAL）的活性，在培养的第28d 以80g/mL浓度的诱导子处理毛葡萄愈伤组织效果最好。不同真菌来源的诱导子对毛葡萄愈伤组织次生代谢的影响效应不同。这些研究结果暗示了通过真菌诱导子调控葡萄次生代谢的可能性。 |
| 英文摘要: |
| Effects of different fungal elicitors on some secondary metabolites of Vitis heyneana callus were studied in this paper. The results indicated that the fungal elicitors can effectively increase the contents of flavonoids and total phenols, and the activities of peroxydase and phenylalnine ammonialyase of the Vitis heyneana cells. The most suitable concentration for elicitors’ treatment is 80 ?g/mL and the appropriate time of treatment is 28 days’ culture of the callus. Elicitors from different species of fungi can cause different physiological effects on Vitis heyneana cells. These results implied the possibility of components controlling of grape by means of fungal elicitors. |
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