| 纪兆林,戴慧俊,张天枢,万保雄,全金成,徐敬友.广西珍珠李叶枯病原鉴定[J].中国南方果树,2015,44(2): |
| 广西珍珠李叶枯病原鉴定 |
| Identification of the pathogen causing leaf blight of plum trees |
| 投稿时间：2014-09-28 修订日期：2014-10-17 |
| DOI： |
| 中文关键词: 李叶枯 致病性 ITS序列 胶孢炭疽菌 |
| 英文关键词:plum leaf blight pathogenicity test ITS sequence Colletotrichum gloeosporioides |
| 基金项目:国家现代农业产业技术体系建设专项 |
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| 中文摘要: |
| 为了明确近年来广西省天峨县发生严重的珍珠李叶枯病原，采集天峨县珍珠李病叶，对病原进行分离和鉴定。本研究从病叶中分离到2种真菌，核糖体DNA内转录间隔区（internal transcribed spacer, ITS）序列分析表明2种真菌分离物分别与GenBank中胶孢炭疽菌（Colletotrichum gloeosporioides）和拟盘多毛孢菌（Pestalotiopsis sp.）具有99%的相似性。离体致病性测定结果表明，分离菌株均能使健康叶片产生症状，并通过柯赫氏法则（Koch’ postulates）验证。结合真菌分离物的分离比率，将广西天峨县珍珠李叶枯病原初步鉴定为胶孢炭疽菌。 |
| 英文摘要: |
| Plum leaf blight is an increasingly serious disease in Guangxi province pearl plum growing areas in recent years. In order to elucidate the causal agent of pearl plum leaf blight in Tian’e county, Guangxi province, this study was carried out to isolate and identify the pathogen. Two kinds of fungi were isolated from all of the diseased leaves of Tian’e pearl plum. The two fungal isolates were characterized with colony morphological characteristics, pathogenicity test and internal transcribed spacer (ITS) analysis of rDNA. The results indicated that the two kinds of fungi belong to Colletotrichum gloeosporioides and Pestalotiopsis sp., respectively. Taking into account the segregation ratio of the two fungal isolates, the pathogen of plum leaf blight was preliminarily identified as anthracnose pathogen C. gloeosporioides. |
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