| 秦永华.无籽沙糖桔高效离体再生体系的建立[J].中国南方果树,2015,44(2): |
| 无籽沙糖桔高效离体再生体系的建立 |
| Establishment of a High Effective Regeneration System for ‘Wuzishatangju’ Mandarin |
| 投稿时间：2015-01-01 修订日期：2015-01-10 |
| DOI： |
| 中文关键词: 无籽沙糖桔，不定芽，再生体系 |
| 英文关键词:Citrus reticulata Blanco, adventitious buds, regeneration system |
| 基金项目:国家自然科学基金项目（面上项目，重点项目，重大项目） |
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| 中文摘要: |
| 本研究以无籽沙糖桔异交种子(无籽沙糖桔×有籽沙糖桔) 的实生苗上胚轴为材料，研究了不同种类的激素及组合对无籽沙糖桔离体再生的影响，建立了高效的无籽沙糖桔离体再生体系。结果表明：ZT和TDZ不利于无籽沙糖桔不定芽的诱导；在一定浓度范围内，随着6-BA浓度的增加，不定芽诱导率和平均每个外植体不定芽数显著增加。当以MT为基本培养基、6-BA浓度为0.25 mg/L时，不定芽诱导率和平均每个外植体不定芽数最高，分别为88.5%和2.7个/外植体；当不同浓度的6-BA与不同浓度的IBA组合时，以6-BA 0.25 mg/L + IBA0.25 mg/L为最佳激素组合，不定芽诱导率达95.2%，平均每个外植体萌芽数为3.6个；再生的不定芽在附加IBA 1.0 mg/L的MT 培养基中生根并再生植株。 |
| 英文摘要: |
| In this work, a high effective regeneration system in-vitro was established for ‘Wuzishatangju’ mandarin by using epicotyls of ‘Wuzishatangju’ seedling (Wuzishatangju× Shatangju) as explants. Effect of hormones on regeneration of ‘Wuzishatangju’ explants was evaluated in considerably detail. ZT and TDZ were not suitable for adventitious bud induction of ‘Wuzishatangju’. 6-BA alone could induce adventitious buds. A high regeneration frequency (88.5%) and 2.7 shoots per explant were obtained on the MT medium supplemented with 6-BA 0.25 mg/L. Our results showed that the optimal hormone supplement for shoot regeneration from epicotyls of ‘Wuzishatangju’ seedling (Wuzishatangju×Shatangju) was 6-BA 0.25 mg/L with IBA 0.25 mg/L, which could produce a high regeneration frequency (95.2%) and 3.6 shoots per explant. The rooting media was MT supplemented with IBA 1.0 mg/L. |
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