| 张俊祥,吴建圆,冀志蕊,迟福梅,徐成楠,周宗山.葡萄炭疽病菌T-DNA插入突变体的表型及致病性变异分析[J].中国南方果树,2015,44(5): |
| 葡萄炭疽病菌T-DNA插入突变体的表型及致病性变异分析 |
| Phenotype and pathogenicity analysis of T-DNA insertional mutants in Colletotrichum gloeosporioides |
| 投稿时间：2015-04-20 修订日期：2015-04-20 |
| DOI： |
| 中文关键词: 炭疽 葡萄 T-DNA 突变体 |
| 英文关键词:Anthracnose Grape T-DNA Mutant |
| 基金项目:中国农业科学院科技创新工程项目（CAAS-ASTIP-2015-RIP） |
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| 中文摘要: |
| 胶孢炭疽菌(Colletotrichum gloeosporioides)引起的葡萄炭疽病是葡萄生产中的一种重要病害。本研究对从2100个T-DNA插入突变体库中挑选的11株PDA培养特征变异较大的突变体进行表型和致病性分析，旨在为葡萄炭疽病菌产孢与致病基因网络调控研究奠定基础。结果表明：突变体M112、M247、M1288、M1325、M1386和M1430不能产生分生孢子，其余的5株突变体产孢量降低；突变体M247、M1288和M1325丧失了致病性；突变体M112、C1094、M1386和M2013突变体致病力增强。此外，突变体C1094孢子萌发率明显降低，其余4株产孢突变体的孢子萌发率与野生型菌株WS15无明显差异。 |
| 英文摘要: |
| The grape anthracnose caused by Colletotrichum gloeosporioides is an important disease in grape production. In this study, 11 isolates selected from T-DNA insertion mutant library including 2100 strains according to variation of cultural characteristics on the PDA medium, and was determined by PCR amplification and green fluorescent detection, and also was performed with phenotypic and pathogenicity analysis. This study will lay the foundation for the study of gene regulatory networks in conidiation and pathogenicity. The result showed as followed：the mutants M112, M247, M1288, M1325, M1386 and M1430 of 11 isolates lost the ability of sporulation, conidia production of remaining isolates decreased significantly; the mutants M247, M1288 and M1325 lost the ability of pathogenicity; virulence of the mutants M112, C1094, M1386 and M2013 increased significantly; conidia germination the of mutant C1094 decreased significantly, however, no difference was observed between conidia germination of remaining 4 mutants producing conidia and the wild isolate WS15. |
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