| 陈华蕊.维多利亚菠萝组培快繁技术研究[J].中国南方果树,2016,45(6): |
| 维多利亚菠萝组培快繁技术研究 |
| Tissue culture and rapid propagation technology |
| 投稿时间：2015-12-27 修订日期：2016-03-29 |
| DOI： |
| 中文关键词: 菠萝 冠芽 诱导 组培 增殖 快繁 |
| 英文关键词:Pineapple crown bud induction Tissue culture multiplication rapid propagation |
| 基金项目:海南省工程技术研究中心专项项目资助项目“菠萝新品种选育及其栽培技术集成与示范推广”；国家科技基础条件平台-国家农作物种质资源平台的子平台“国家热带作物种质资源平台/NICGR2015-067”；公益性行业科研专项“菠萝产业技术研究与试验示范”（201203021） |
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| 中文摘要: |
| 以“维多利亚”菠萝品种的冠芽为外植体，采用6－BA、NAA、IBA等激素配比对芽诱导、愈伤诱导、继代增殖和生根培养。结果表明， 愈伤组织诱导率最高的为MS BA2.0 mg/L NAA0.2 mg/L。愈伤诱导不定芽最理想配比为MS 6-BA3 mg/L NAA0.1 mg/L。芽的继代增殖系数较高和芽较粗壮的培养基配比为MS 6-BA2.0 mg/L NAA0.1 mg/ L，适宜生根培养基为MS＋IBA0.5 mg/L＋NAA 1 mg/L。生根率达100%。 |
| 英文摘要: |
| The crown bud of pineapple variety Victoria was used as explants, different hormone combinations rang-ing of 6-BA, NAA and IBA were added to MS or medium to conduct the induction of bud, Callus induction， subculture of cluster buds and rooting of plantlets. The results showed that MS 6-BA (2.0 mg/L) NAA(0.2 mg/L) was the best medium for the induction of Callus induction, The most suitable adventitious bud medium was MS 6-BA(3 mg/L) NAA(0.1 mg/L)，MS 6-BA (2.0mg/L) NAA(0.1mg/L) can made the proliferation times of Subculture multiplication of buds was better and Buds grow strong.The optimal medium for rooting was MS IBA(0.5 mg/L) NAA(1 mg/L) with rooting rate of 100%. |
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