| 高恒锦,王小柯,张琰,闫树堂,孙珍珠,江东.利用SSR和Indel标记对椪柑的遗传多样分析[J].中国南方果树,2016,45(4): |
| 利用SSR和Indel标记对椪柑的遗传多样分析 |
| The Genetic Diversity Analysis of Ponkan Using SSR and Indel Molecular Marker |
| 投稿时间：2016-02-29 修订日期：2016-02-29 |
| DOI： |
| 中文关键词: 分子标记 椪柑 分类 |
| 英文关键词:Molecular marker, ponkan,classification |
| 基金项目:“十二五”国家科技支撑计划项目（2013BAD01B04-22）；公益行业专项“重要果树野生资源的农艺及品质性状评价与发掘利用”(201303093) |
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| 中文摘要: |
| 本研究利用SSR和InDel分子标记对26份来自不同国家与地区的椪柑资源进行了遗传多样性分析。21对引物共获得62个标记位点，产生了105条多态性条带，平均每对引物检测到5条扩增条带。多态性标记位点的平均香农信息指数为0.32，平均期望杂合度为0.30。群体遗传结构和主坐标轴分析均表明，椪柑资源内部遗传多样性水平低，遗传变异较小。印度酸桔与椪柑遗传关系较远，Emperor、濑户见及早香等椪柑杂交后代，与椪柑存在明显遗传差异，通过不同的分子标记组合可以有效鉴别区分椪柑材料。 |
| 英文摘要: |
| In this research SSR and InDel markers were used to analyze genetic diversity of 25 ponkan germplasm resources. 21 pairs of primers produced 62 marker loci and 105 polymorphism bands, on average 5 amplified bands were detected on each pair of primers. The mean of Shannon index of primers was 0.32 and average expected heterozygosity was 0.30. Population genetic structure and principal axis analysis indicates ponkan showed a low genetic diversity among different accessions. Cleopatra sunki have far phylogenetic relationship with ponkan , Emperor 、Setomi and Hayaka mandarin as hybrid offspring of ponkan showed a distinct differences from ponkan. Although genetic diversity among different ponkan cultivars is low, different primer combination could be used to distinguished them from each other. |
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