| 尹锦辉,李化强.酶法去除脐橙白皮层的产酶培养基优化研究[J].中国南方果树,2016,45(5): |
| 酶法去除脐橙白皮层的产酶培养基优化研究 |
| Study on optimization of medium for fermentation of enzymatic removal of albedo in citrus |
| 投稿时间：2016-07-16 修订日期：2016-07-20 |
| DOI： |
| 中文关键词: 脐橙 培养基 果胶酶 |
| 英文关键词:Navel orange Medium Pectinase |
| 基金项目:国家公益性行业科研专项（201405003-3），湖南省科技厅重点研发计划项目 （2015CK3031、13CY028），湖南省科技厅创新平台与人才计划项目（2015TP2022），湖南省教育厅科学研究一般项目（14C1022） |
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| 摘要点击次数: 2012 |
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| 中文摘要: |
| 本研究通过对酶法脱脐橙白皮层的微生物培养基成分进行优化，测定发酵液中果胶酯酶（PE）、果胶裂解酶（PL）、聚半乳糖醛酸酶（PG）活力，探讨碳源量、氮源种类和含量对3种果胶酶活性的影响。单因素和响应面优化试验结果表明：在氮源硝酸铵:硫酸铵=3.02:1、氮源添加量为8.52 g/L、碳源添加量为9.26 g/L时，PG酶活为497.26 U/mL，PL酶活为461.05 U/mL，PE酶活为40.42 U/mL，提升PG酶活141.83%，PL酶活46.85%，PE酶活58.14%，去除白皮层效果提升显著，为发展环境友好型橙汁胞加工工艺提供参考。 |
| 英文摘要: |
| In the process of enzymatic de-sac endocarp in citrus, the fermentation medium optimized to explore amount of carbon, nitrogen sources and types of content by determine pectinase activity. The results show that: in nitrogen ratio was 3.02 (ammonium nitrate / ammonium sulfate), nitrogen dosage 8.52 g / L, carbon dosage 9.26 g /L , the PG activity was 497.26 U / mL, PL activity was 461.05 U / mL, PE activity was 40.42 U / mL. By study on optimization of medium for fermentation of enzymatic de-sac endocarp in citrus, research on it effect of the PE, PL, PG, in order to have some guidance for enzymatic de-sac endocarp citrus production application and promotion. |
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