| 刘恋,谢深喜,刘翠,吴倩,卢晓鹏.温州蜜柑果实汁胞特征及其离体培养发育规律初探[J].中国南方果树,2017,46(1): |
| 温州蜜柑果实汁胞特征及其离体培养发育规律初探 |
| Primary Studies on Characteristics and Cultivation in Vitro of Satmuma Juice Sacs |
| 投稿时间：2016-08-01 修订日期：2016-10-28 |
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| 中文摘要: |
| 为了解柑橘果实品质形成规律，本研究以大浦5号温州蜜柑为材料，开展了温州蜜柑果实汁胞特征分析和离体培养体系的探索。结果表明，温州蜜柑汁胞原始形态表现出有尾较长和无尾较短两种形态，两种汁胞在大果和小果中所占比例有差异。在各糖酸组分含量方面，大果汁胞蔗糖含量稍低于小果，大果有尾汁胞柠檬酸含量低于其他类型汁胞，而其苹果酸含量高于其他类型。温州蜜柑汁胞在暗培养条件下随培养时间延长逐渐着色，培养20天后明显着色，30天后完全着色或褐化。汁胞着色过程中有尾汁胞始终先于无尾汁胞着色，较高浓度蔗糖培养着色较快。随培养时间延长，汁胞长度在培养20天内持续增加，20天后持续降低，60g/L和80g/L蔗糖浓度培养下汁胞生长量显著小于40g/L。汁胞暗培养过程中，其蔗糖含量呈先降低后升高的趋势，而果糖和葡萄糖呈持续升高的趋势。不同处理间，汁胞蔗糖含量以80g/L蔗糖处理升高最快，40g/L和60g/L处理升高较慢。汁胞暗培养10天后，不同糖浓度处理柠檬酸和苹果酸含量均迅速下降， 60g/L和80g/L蔗糖处理酸下降速度较40g/L慢。综上，温州蜜柑果实有尾和无尾汁胞品质差异不大，离体培养时间以不超过20天为宜，汁胞生长速度与培养基蔗糖含量明显相关，以培养基蔗糖浓度80g/L培养汁胞最接近生产中糖酸积累模式。 |
| 英文摘要: |
| Aiming to understand formation of citrus fruit quality, characteristics of satsuma juice sacs and its cultivation system in vitro were studied using \"Dapu No.5\" (Citrus unshiu) as materials. The results showed that satsuma juice sacs exhibit two types, tailed and tailless juice sacs. There were differences in portion of tailed and tailless juice sacs in both big and small size fruits. Totally, juice sacs in big size fruit contained less sucrose compared to that in small size fruit; the tailed juice sacs in big size fruit showed the lowest citrate and highest malate contents.
In dark cultivation-condition, satsuma juice sacs gradually colored with significant color-change after 20 days and completely coloring or browning after 30 days. During juice sac coloring process, tailed juice sacs colored always earlier than tailless juice sacs; juice sacs which were cultivated on medium with more sucrose colored always earlier than juice sacs on less sucrose medium. During cultivation, the length of juice sacs continued to increase within 20 days but continuously decreased after 20 days. Prolongation of juice sacs on 40g/L sucrose medium was more than that on 60 and 80g/L sucrose mediums. Under dark cultivation, sucrose in juice sacs decreased at first and then increased, while fructose and glucose rose constantly. Between different treatments, sucrose in juice sacs increased fastest on 80g/L sucrose medium and that on 40 and 60g/L sucrose mediums followed. After juice sacs cultivating for 10 days, citrate and malate contents rapidly declined in each treatment which decreased fastest in 40g/L sucrose treatment. In summary, tailed and tailless juice sacs showed no significantly different quality. It is an optimum cultivated period within 20 days. Growth of juice sacs significantly associated with sucrose content in medium and sugar and acid accumulations in juice sacs cultivated on 80g/L sucrose medium were closest to that in field. |
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