| 柏自琴,王雪峰,罗怿,罗会,陈守一,李金强.贵州柑桔主产区柑桔黄龙病菌发生及遗传多样性研究[J].中国南方果树,2017,46(3): |
| 贵州柑桔主产区柑桔黄龙病菌发生及遗传多样性研究 |
| Occurrence and genetic variation of ‘Candidatus Liberibacter’ in main production areas of Guizhou Province |
| 投稿时间：2017-01-09 修订日期：2017-02-25 |
| DOI： |
| 中文关键词: 贵州省 柑桔黄龙病 PCR检测 遗传变异 |
| 英文关键词:Guizhou Citrus Huanglongbing PCR detection Genetic variation |
| 基金项目:高低海拔地区柑橘黄龙病病原菌遗传特性差异研究（黔科合LH字【2015】7084号）；国家现代农业柑桔产业技术体系贵州特色柑橘综合试验站项目；从江椪柑品质提升关键技术集成与示范(黔科合成转字【2014】5046号) |
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| 中文摘要: |
| 为进一步调查贵州柑桔黄龙病（Citrus Huanglongbing，HLB）疫情，本研究在贵州15个县（市）采集了104份柑桔黄龙病疑似样品，从中检测出19份样品感染了该病，阳性检出率为18.27%。贵州柑桔黄龙病发生仍以黔西南地区的老病区为主，从江、榕江等黔东南地区疫情得到一定控制，黔南、遵义及铜仁等地区没有发现疫情。通过特异引物扩增贵州省内柑桔黄龙病病原菌种群发现，产物呈现扩增多态性，表明贵州柑桔黄龙病菌种群在遗传特性存在差异。 |
| 英文摘要: |
| To further investigate the occurrence of Citrus Huanglongbing(HLB) in Guizhou citrus main production areas, 104 HLB-suspected leaf samples obtained from 15 counties were analyzed by PCR detection. The results showed that 19 samples were HLB positive, and HLB occurred still in some old orchards in Southwest Guizhou. HLB was controlled in Southeast Guizhou such as Congjiang and Rongjiang county, and HLB was not found in South, Zunyi and Tongren region. Populations of ‘Candidatus Liberibacter’ in Guizhou province were tested by a pair of specific primer, the results suggested that the genetic differences were found among ‘Ca. L.’ in Guizhou province. |
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