| 张丹华,张红楠,吴頔,梁国鲁.引致枇杷叶斑病的链格孢菌的鉴定及其化学防治药剂筛选[J].中国南方果树,2017,46(4): |
| 引致枇杷叶斑病的链格孢菌的鉴定及其化学防治药剂筛选 |
| Identification of The Pathogen Causing Loquat Leaf-spot Disease and The Screening of Fungicides |
| 投稿时间：2017-01-10 修订日期：2017-02-28 |
| DOI： |
| 中文关键词: 枇杷 叶斑病 链格孢菌 杀菌剂 毒力测定 |
| 英文关键词:loquat leaf-spot disease Alternaria alternate fungicides toxicity test |
| 基金项目:国家十二五计划项目（2013BAD02B02）；西南大学基本科研业务费专项资金项目（XDJ2016185）；中国博士后科学基金（2016M592621）面上资助。 |
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| 中文摘要: |
| 结合形态学与分子生物学分析，对采自重庆地区疑似由链格孢菌引起的枇杷叶斑病样品进行病原菌分离鉴定。结果表明，所得病原菌的rDNA-ITS序列与 GenBank中登陆的四株链格孢菌Alternaria alternate（登录号分别为：FJ228163、KT192329、KM100451、KP278185）的相似性均为99%，结合病原菌的菌落、分生孢子形态特征及致病性测定结果，将其鉴定为链格孢菌Alternaria alternate，GenBank登录号为KY397985，并命名为24-CQYB。室内毒力测定结果显示，咪鲜胺、嘧菌酯、甲基硫菌灵、多菌灵及苯醚甲环唑等5种杀菌剂对24-CQYB菌株生长均有抑制作用，其中苯醚甲环唑的抑制效果最好，EC50为3.84 mg/L。 |
| 英文摘要: |
| The strains were isolated from the symptomatic leaves collected in Chongqing Province of China and further identified at morphological and molecular level. The results showed that the rDNA-ITS sequences of the isolate (GenBank No. KY397985) shared 99% identity with those previously reported Alternaria alternate strains (corresponding accession numbers were FJ228163, KT192329, KM100451 and KP278185) on other hosts. Additionally, the 24-CQYB labeled strain was identified as Alternaria alternate according to the morphological characteristics of colony and conidia, and the results of pathogenicity test. Furthermore, the toxicity test showed that 24-CQYB was suppressed by prochloraz，azoxystrobin，thiophanate-methyl，carbendazim and difenoconazole. Difenoconazole was the most effective fungicide control treatment, and the mean effective concentration (EC50) value was 3.84 mg/L. |
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