| 张伟清,林媚,王天玉,冯先桔.不同贮藏期柑橘Vc含量HPLC法测定的建立及应用[J].中国南方果树,2018,47(3): |
| 不同贮藏期柑橘Vc含量HPLC法测定的建立及应用 |
| Establish of a method of determination of Vc in citrus during storage by HPLC and application |
| 投稿时间：2017-08-14 修订日期：2018-02-28 |
| DOI： |
| 中文关键词: 高效液相色谱 椪柑 浅色水果 Vc |
| 英文关键词:Key words: high performance liquid chromatography(HPLC) ponkan light-colored fruits Vitamin C |
| 基金项目: |
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| 摘要点击次数: 1959 |
| 全文下载次数: 2259 |
| 中文摘要: |
| 通过对波长、流动相的优化以及Vc稳定性影响试验，建立了HPLC法测定柑橘中Vc含量。采用C18色谱柱分离，以甲醇-磷酸二氢钾磷酸盐缓冲液（0.01mol/L，pH3.5）体积比=8: 92为流动相，流速0.5mL/min，检测波长 245nm，柱温25℃，进样量20uL，所得图谱峰分离效果和峰形都较好。Vc质量浓度在0.5~10mg/L范围内，具有良好的线性关系，回归方程为：y=110.4x-12.33，R2=0.9990。三个不同浓度的加标水平下，Vc的加标回收率分别为98.85%，99.14%和99.48%，相对标准偏差分别为0.54%，2.91%和2.62%。HPLC法通过优化后测定椪柑中Vc含量，与2-6二氯靛酚滴定法相比，结果一致。利用HPLC法对不同贮藏期椪柑中Vc含量进行测定，发现Vc含量随着时间的增加而减少，从30.15mg/100g经储藏84d后下降为18.87mg/100g，建议鲜食柑橘果实Vc营养价值更高。本试验建立的HPLC法具有前处理简单、快速、重现性好、准确度和回收率高等优点，同样适用于如温州蜜柑、本地早、瓯柑、葡萄、枇杷、猕猴桃等其它浅色水果中Vc含量的测定。 |
| 英文摘要: |
| Through the optimization of wavelength and flow phase and the study of stability of Vc, a new method of determination of Vc in citrus by HPLC was established.The analytes were separated on a C18 column using methanol- potassium dihydrogen phosphate buffter (0.01mol/L，pH 3.5)=( 8: 92) as mobile phase, and analyzed quantitatively at speed of 0.5mL/min with sample injection rate of 20uL ? and wavelength of 245nm with the column temperature at 25℃.The Vitamin C was analyzed with good peak shape and separation effect. The calibration curve for Vitamin C was linear in the range of 0.5~10mg/L with correlation coefficient was 0.9990,and the linear equation was y=110.4x-12.33. The average recoveries were 98.85%,99.14% and 99.48% and the relatively standard deviation were 0.54%,2.91% and 2.62%. The content Vc in ponkan detected by HPLC had no difference with that by 2-6 dichloro-indophenol titration. By analyzing the change of the Vc content in ponkan by HPLC during storage, the Vc content decreased from 30.15mg/100g to 18.87mg/100g with the incerse of time and it was suggested that the nutritive value of fresh citrus fruits were higher. The results showed that HPLC was simple and rapid with good reproducibility, precise and reliable, and also suitable for the determination of Vc in other light-colored fruits such as satsuma mandarin, bendizao,ougan, grape,loquat and kiwifruit. |
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