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余贤美,侯长明,王洁,张坤鹏,艾呈祥.柿树炭疽病菌的生物学特性及其抑菌药剂筛选[J].中国南方果树,2018,47(2):
柿树炭疽病菌的生物学特性及其抑菌药剂筛选
Biological Characteristics of Persimmon Anthracnose Pathogen Colletotrichum horii and Screening of Inhibitory Fungicides
投稿时间:2017-09-19  修订日期:2017-11-10
DOI:
中文关键词:  柿树炭疽病  哈锐炭疽菌  生物学特性  抑菌药剂  室内药剂筛选
英文关键词:Persimmon anthracnose  C.horri  Biological characteristics  Inhibitory fungicides  Fungicides screening in laboratory
基金项目:“十二五”农村领域国家科技计划项目;山东省农业良种工程项目;山东省农业科学院青年科研基金项目
作者单位E-mail
余贤美 山东省果树研究所 yuxianmei95@163.com 
侯长明 山东省农业科学院 sdlyhcm@163.com 
王洁 山东省果树研究所 jiewangsdau@163.com 
张坤鹏 山东省果树研究所 zkp200886@163.com 
艾呈祥* 山东省果树研究所 chengxiang_1975@163.com 
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中文摘要:
      通过十字交叉法和血球计数板法测定柿树炭疽病菌Colletotrichum horri的生物学特性,采用菌丝生长速率法和孢子萌发法分别测定16种常用杀菌剂对该菌菌丝生长的抑制作用和对孢子萌发的影响。柿树炭疽病菌菌丝生长的最适温度为25篊,最适产孢温度为28篊,适合菌丝生长的pH值为4.0~6.0,最适产孢pH值为4.0;最佳碳源为葡萄糖和麦芽糖,最佳氮源为牛肉膏。16种常用杀菌剂中, 33.5%喹啉铜悬浮剂、25%溴菌腈乳油和70%代森锰锌可湿性粉剂对柿树炭疽病菌菌丝生长和产孢的抑制效果最好,可作为防治柿树炭疽病的优选药剂,其次为70%代森联水分散粒剂、400 g?L-1氟硅唑乳油和50%福美双可湿性粉剂,可作为备选药剂。该研究结果可为柿树炭疽病的有效防治提供试验依据。
英文摘要:
      In this study, the biological characteristics of Colleltrichum horri causing persimmon anthracnose was investigated using the crossing method and blood count plate method, and the inhibitory fungicides were screen via mycelial growth method and spore germination method. The results showed that C.horri grew well at 25oC and produced a large number of spores when inoculated at 28oC using glucose or maltose as the extra carbon resource and using beef extract as the extra nitrogen resource, and when the medium pH value was 4.0. Among the 16 tested fungicides, the fungicides of 33.5% Copper quinolate SC, 25% Bromothalonil EC and 70% Mancozeb WP achieved the best inhibitory effect in mycelial growth and spore germination, which could be used as the preference fungicides for the control of persimmon anthracnose; and 70% Polyram WG, 400 g?L-1 Flusilazole EC and 50% Triram WP achieved the secondly best inhibitory effect, which could be used as alternative fungicides. The results of this research will provide the scientific evidence for the effective control of persimmon anthracnose, and provide more optional pesticides for utilization in the production practice of persimmon industry.
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