| 汤红,张平贤,郭大勇,张青林,罗正荣.柿属植物iPBS标记的建立及优化[J].中国南方果树,2018,47(4): |
| 柿属植物iPBS标记的建立及优化 |
| Establishment and optimization of IPBs markers in Diospyros L. |
| 投稿时间：2017-10-12 修订日期：2018-01-08 |
| DOI： |
| 中文关键词: 柿属植物,iPBS标记,遗传多样性 |
| 英文关键词:Diospyros L. iPBS marker genetic diversity |
| 基金项目:农业部公益性行业科研专项(201203047)；华中农业大学园艺林学学院大学生科技创新(2015YLSRF015) |
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| 摘要点击次数: 1947 |
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| 中文摘要: |
| 试验建立并优化了柿属植物的iPBS(inter Primer Band Site)标记PCR反应体系。最佳反应体系为1x Es Taq MasterMix,60 ng DNA模板,10 μmol/l终引物浓度,总体积12 μL。共筛选出18个多态性较好的引物。基于iPBS标记的UPGMA分析结果与前人一致,iPBS标记更简单、经济,可以用于柿属植物种质资源的多样性评价和种质鉴定。 |
| 英文摘要: |
| In this study, it was established and optimized of Diospyros IPBs (inter primer band site) PCR reaction system. The optimum conditions of 12 μL PCR reaction system in Diospyros were as follows: 6μL 2x Es Taq MasterMix, 60ng DNA template, 10 μmol/l primer. 18 iPBS polymorphic primers were screened for PCR amplification. The results of UPGMA analysis based on IPBs markers consistent with previous. This study provided a new simple and efficiently method for diversity evaluation and identification of germplasm resources in Diospyros L. |
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