| 宋 杨,刘红弟,王海波,张红军,刘凤之.越橘VcWRKY33基因的分离及其响应几种胁迫的表达分析[J].中国南方果树,2018,47(5): |
| 越橘VcWRKY33基因的分离及其响应几种胁迫的表达分析 |
| Cloning and Expression Analysis of blueberry VcWRKY33 Gene in Response to Stress |
| 投稿时间：2018-07-26 修订日期：2018-07-26 |
| DOI： |
| 中文关键词: 越橘 WRKY基因 抗逆性 |
| 英文关键词:blueberry WRKY gene stress tolerance |
| 基金项目: |
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| 摘要点击次数: 1895 |
| 全文下载次数: 2186 |
| 中文摘要: |
| 本研究以南高丛越橘品种‘雷格西’为试材,克隆抗逆相关基因WRKY,分析其表达模式及其对高盐、低温、PEG6000和水杨酸处理的响应。结果表明,克隆获得越橘VcWRKY33基因,含有2个WRKYGQK结构域和1个C2H2锌指结构。基因表达分析结果表明,VcWRKY33在不同组织器官中均可表达,但表达量差异明显,在叶片中表达量较高。该基因明显受高盐、低温、PEG6000和水杨酸诱导表达。VcWRKY33在越橘的抗盐及抗寒等过程中可能起重要作用。 |
| 英文摘要: |
| This study is aiming at cloning WRKY gene related to stress from southern highbush blueberry (Vaccinimu corymbosum ‘Legacy’), to analyze expression pattern and exposing to high salinity, cold, PEG6000 and salicylic acid (SA). VcWRKY33 gene was obtained. Sequence analysis revealed that VcWRKY33 exhibited two WRKYGQK and C2H2 domain. The real time qRT-PCR analysis indicated that VcWRKY33 was expressed in different organ. The expression of VcWRKY33 showed high level in leave and induced by high salinity, cold, PEG6000 and SA. VcWRKY33 may play an important role in salinity and cold tolerance. |
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