| 娄兵海,白先进,陈爱军,白扬,宋雅琴,王博,王明召,张敏,何建军,刘萍.广西葡萄良种脱毒研究进展[J].中国南方果树,2019,48(2): |
| 广西葡萄良种脱毒研究进展 |
| Progresses in Research on Virus Elimination of Grapevine in Guangxi |
| 投稿时间：2018-09-18 修订日期：2018-11-15 |
| DOI： |
| 中文关键词: 葡萄 RT-PCR检测 热处理 茎尖培养 脱毒 |
| 英文关键词:grapevine RT-PCR detection thermotherapy shoot tip culture virus elimination |
| 基金项目:广西科学研究与技术开发计划项目(桂科合15104001-19)；广西壮族自治区八桂学者建设工程专项项目(2011A019)；国家现代农业产业技术体系广西特色水果创新团队(nycytxgxcxtd-04-19-4)；广西创新驱动发展专项资金项目(桂科AA17204097) |
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| 中文摘要: |
| 建立葡萄脱毒技术并繁育和推广葡萄良种脱毒苗木,对于广西葡萄产业的健康可持续发展极为重要。本研究团队在广西最先开展了葡萄脱毒技术研究。目前已建立了10种葡萄主要病毒的RT-PCR检测方法,并建立了热处理结合茎尖培养的葡萄脱毒技术,且已成功对‘阳光玫瑰’葡萄、‘夏黑’葡萄和‘巨峰’葡萄三个优良品种的带毒单株进行了脱毒。研究成果有助于进一步推动广西葡萄产业的健康可持续发展。 |
| 英文摘要: |
| Development of virus elimination method and propagation of virus-eliminated nursery stocks are important to the healthy and sustainable development of grape industry in Guangxi. We first carried out research on virus elimination of grapevine in Guangxi and had achieved some important progress, including the successful development of RT-PCR detection methods for ten grapevine viruses, the successful development of virus elimination of grapevine by thermotherapy combined with shoot tip culture and the successful virus elimination of virus-infected plants of three grapevine varieties, "Shine Muscat", "Kyoho" and "Summer Black", respectively. These results can help to promote the further development of grape industry in Guangxi. |
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