| 周彩霞,黄桂香,潘贞珍,胡艺帆,苏美玲,何新华.甜橙无核变异品系（春蜜）的亲缘关系分析[J].中国南方果树,2019,48(3): |
| 甜橙无核变异品系（春蜜）的亲缘关系分析 |
| Analysis on Genetic Relationship of Seedless Variant of the Sweet Orange(Chunmi ) |
| 投稿时间：2019-01-06 修订日期：2019-02-24 |
| DOI： |
| 中文关键词: 甜橙 无核变异 叶片性状 SCoT标记 亲缘关系 |
| 英文关键词:Sweet orange Seedless variation Leaf morphology SCoT marker Phylogenetic relationship |
| 基金项目:特色果蔬、花卉优异种质资源挖掘与品种选育（合同编号：桂科AA17204026-2）；广西现代农业产业技术体系柑橘创新团队南宁综合试验站（合同编号：nycytxgxcxtd-05-08） |
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| 中文摘要: |
| 以甜橙无核变异品系（春蜜）和41份甜橙为试材，通过叶片形态鉴定和SCoT分子标记分析甜橙无核变异品系（春蜜）与41份柑橘的亲缘关系。结果表明：在叶片描述中，叶片的数量性状变异系数最大的三个性状是叶重（26.72%）、翼叶的长（22.48%）和宽（36.56%）；通过对叶片表型性状描述的R型聚类分析，得出适用于甜橙叶片表型性状描述的指标为叶身形、叶尖形、叶基形、叶缘、叶尖缺刻有无、叶形指数、叶重、叶厚度、叶柄长、翼叶长和翼叶宽；在Q型聚类中，无核变异品系和春橙距离最近，在欧氏距离为1时聚在一组。在SCoT标记中，42份资源的平均多态位点比率为65.26%，在遗传系数为0.938处，无核变异品系与春橙聚到一组；在遗传系数为0.91处，无核变异品系与春橙以及埃及糖橙分到一组；在遗传系数为0.831处，无核变异品系与新会橙等甜橙分在一组；在遗传系数为0.819处，无核变异品系则与春橙、埃及糖橙、哈姆林、暗柳橙、冰糖橙和桂橙1号等普通甜橙分在一组。综合分析认为，甜橙品种的叶片形态可作为甜橙品种分类的基本依据之一；叶片形态标记和SCoT分子标记相结合能较明确该品系的亲缘关系，该甜橙无核变异品系属于普通甜橙类，很可能源于春橙的无核优质芽变。 |
| 英文摘要: |
| The experiment aimed to analyze the relationship among the seedless variant of sweet orange and 41 sweet oranges by means of leaf morphological description and SCoT molecular marker. The results indicated that in leaf morphology, the leaf weight（26.72%）, the petiole wing length (22.48%) and petiole wing width (36.56%)were the three characters with the highest coefficient of variation. The applicable indicators for the description of leaf phenotypic characteristics of sweet orange leaves are leaf shape, leaf tip shape, leaf base shape, leaf edge, having leaf tip deficiency or not, leaf shape index, leaf weight, leaf thickness, petiole length, the length and width of petiole wing based on the R-type clustering analysis of leaf phenotypic traits. In the Q-type cluster analysis, the seedless variant and Chuncheng was closest to each other and gathered in a group when the Euclidean distance was 1. In SCoT marker, the average polymorphic locus ratio of 42 resources was 65.26%, the seedless variant was grouped with Chuncheng at the genetic coefficient of 0.938, the seedless variant was divided into a group with Chuncheng and Succari Orange at the genetic coefficient of 0.91, the seedless variant was grouped with Citrus sinensis Osbeck var. Sunwui-tincheng. Ex Hort at the genetic coefficient of 0.831, and the seedless variant was grouped with common sweet oranges such as Chuncheng, Succari Orange, Citrus sinensis(L.) Osbeck,Citrus sinensis Osbeck cv. ‘Anliu’, Bingtang Sweet Orange and Guicheng No.1 Orange at the genetic coefficient of 0.819. Based on above results, leaf morphology of sweet oranges can be used as one of the basic basis for classification of sweet orange varieties.Combining leaf morphological markers and SCoT molecular markers can further clarify the genetic relationship of the the seedless variant of sweet orange which is a common sweet orange and is probably derived from the seedless and high quality germination of Chuncheng. |
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