| 郭丽霞,张燕玲,蓝福生,张明良,张川梅,冯良军,张馨丹.罗汉果花粉超低温贮藏及其生活力测定[J].中国南方果树,2020,49(1): |
| 罗汉果花粉超低温贮藏及其生活力测定 |
| The Pollen Cryopreservation and the Viability Test of siraitia grosvenorii |
| 投稿时间：2019-03-29 修订日期：2019-06-05 |
| DOI： |
| 中文关键词: 罗汉果 花粉 超低温保存 生活力测定 |
| 英文关键词:siraitia grosvenorii pollen cryopreservation Viability Test |
| 基金项目:桂林市科技攻关项目“医药及生物技术新产品、新技术研发与应用”—— 罗汉果活性物质提取技术研究及产品开发-罗汉果花粉超低温贮藏技术与花粉库建立(2016010304-1)。 |
|
| 摘要点击次数: 2041 |
| 全文下载次数: 2517 |
| 中文摘要: |
| 研究了罗汉果花粉在超低温保存条件下的花粉寿命,并对罗汉果花粉的超低温保存技术程序进行了研究。结果表明：罗汉果超低温保存的程序为新鲜花粉脱水(低于含水量30%)后密封保存在超低温冰箱或液氮(LN)中,结束保存时采用冷藏、室温逐级化冻花粉,授粉前使用MTT染色法快速鉴定花粉生活力。 |
| 英文摘要: |
| This paper focuses on the effects of cryopreservation on the pollen of siraitia grosvenorii and provides a promising cryopreservation procedures. The results showed that the procedure of cryopreservation of siraitia grosvenorii was the fresh pollen was dehydrated by anhydrous calcium chloride for 72 h (below 30% water content) and then sealed and stored in ultra-low temperature refrigerator (-86 ℃ ). At the end of storage, the frozen pollen was thaw out step by water bath(38℃) before use, and MTT staining can be used to test pollen viability quickly before pollination. It was simple, low cost and high pollen survival rate dealing with the method above. It will be used for large-scale planting of Siraitia grosvenorii with spray pollination technology. |
| 查看/发表评论 下载PDF阅读器 |
| 关闭 |
