| 柏自琴,王小柯,冯建文,李金强,罗会,罗怿,李兴忠,李文云,马玉华.贵州柑桔黄龙病菌两个原噬菌体位点遗传多样性分析[J].中国南方果树,2019,48(5): |
| 贵州柑桔黄龙病菌两个原噬菌体位点遗传多样性分析 |
| Genetic variation analysis of Guizhou ‘Candidatus Liberibacter asiaticus’ on two prophage-related loci |
| 投稿时间：2019-05-31 修订日期：2019-06-20 |
| DOI：10.13938/j.issn.1007-1431.20190316 |
| 中文关键词: 贵州省 柑桔黄龙病 原噬菌体 遗传变异 |
| 英文关键词:Guizhou Citrus Huanglongbing Prophage Genetic variation |
| 基金项目:国家重点研发计划项目（2018YFD0201508）；贵州省科学技术厅与贵州省农业科学院联合基金项目（黔科合LH字【2015】7084号）；现代农业产业技术体系建设专项资金资助（CARS-26） |
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| 中文摘要: |
| 为研究贵州柑桔黄龙病菌（‘Candidatus Liberibacter asiaticus’）遗传多样性，分析了54份病原菌株系在两个原噬菌体位点上的变异情况。结果显示不同地理来源病原菌株系在两位点上扩增情况不同，扩增条带之间序列亦存在碱基的变异及序列的插入/缺失；不同海拔区域原噬菌体类型及组合的情况不同；结合同源性分析，得出贵州南部‘Ca. L. asiaticus’ 菌群存在2种或多种原噬菌体类型的可能，田间存在不同菌系混合侵染，来源相对复杂。 |
| 英文摘要: |
| 54 samples of ‘Candidatus Liberibacter asiaticus’ from 6 counties (cities) in southern Guizhou were used to study the genetic variation of pathogenic bacteria on two prophage-related loci. Results showed that PCR amplicon polymorphisms and different amplicon types were observed. The sequence differences were mainly caused by nucleotides insertion/deletion and mutation. The types and combinations of prophages were different based on altitudinal gradient. Combined homology analysis, it is concluded that there are two or more prophage types in ‘Ca. L. asiaticus’ strains collected from southern Guizhou. Mixed infection of different strains exist in the field, and its sources are relatively complex. |
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