| 张安世,张中海,高登涛,魏志峰.基于iPBS的巨峰系葡萄品种遗传多样性分析及MCID法快速鉴定[J].中国南方果树,2020,49(4): |
| 基于iPBS的巨峰系葡萄品种遗传多样性分析及MCID法快速鉴定 |
| Genetic%20diversity%20analysis%20and%20rapid%20identification%20by%20MCID%20method%20for%20Kyoho%20grapevine%20series%20germplasm%20based%20on%20iPBS%20markers |
| 投稿时间：2019-10-25 修订日期：2019-12-03 |
| DOI：10.13938/j.issn.1007-1431.20190606 |
| 中文关键词: 葡萄 %20iPBS %20遗传多样性 人工绘制植物品种鉴别图(MCID)法 |
| 英文关键词:Grape %20iPBS %20genetic%20diversity %20manual%20cultivar%20identification%20diagram(MCID) |
| 基金项目:中国农业科学院科技创新工程专项经费项目(CAAS-ASTIP-2015-RIP -04) |
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| 中文摘要: |
| 为探讨巨峰系葡萄种质的遗传关系,利用iPBS标记对24个巨峰系葡萄品种进行遗传多样性分析,并应用人工绘制品种鉴别示意图法(%20MCID法)建立品种鉴定图(CID)%20。筛选出13个引物对24个巨峰系葡萄品种进行PCR扩增,共扩增出136条带,其中多态性条带99条,多态性比率为72.79%。各引物观测等位基因数(Na)、有效等位基因数(Ne)、Nei’s基因多样性指数(H)和Shannon’s信息指数(I)的平均值分别为1.7206、1.2971、0.1874和0.2972。24个巨峰系葡萄品种的遗传相似系数(GS)在0.6618~0.9485之间,变幅为0.2867。上述结果表明：24个巨峰系葡萄品种间的遗传差异较小。利用UPGMA构建24个巨峰系葡萄种质资源的聚类树状图,在遗传相似系数为0.74处可将24个巨峰系葡萄品种分为2组,其中第Ⅰ组有22个品种,均为欧美杂交种；第Ⅱ组有2个品种,分别为山欧杂种和欧亚种。利用5条引物扩增的多态性谱带构建了24个巨峰系葡萄品种的CID图,利用此CID图可以找出区分任意2个品种的引物,为巨峰系葡萄品鉴定提供科学依据。 |
| 英文摘要: |
| In%20order to explore%20the%20genetic%20relationship%20among%20the%20germplasm%20resources%20of%20Kyoho%20grapevine%20series%20,%20the%20genetic%20diversity%20of%2024%20cultivars%20was%20analyzed%20by%20iPBS%20marker,%20and%20the%20cultivar%20identification%20diagram%20(CID)was%20constructed%20by%20using%20the%20manual%20cultivar%20identification%20diagram%20(MCID).13%20primers%20were%20screened%20for%20PCR%20amplification%20of%2024%20Cultivars.%20A%20total%20of%20136%20bands%20were%20generated,%20of%20which%2099%20bands%20were%20polymorphic%20bands(72.79%).The%20average%20value%20of%20observed%20number%20of%20alleles(Na),%20effective%20number%20of%20alleles(Ne),%20Nei’s%20gene%20diversity%20(H)%20and%20Shannon’s%20information%20index%20(I)%20were%201.7206,%201.2971,%200.1874%20and%200.2972,%20receptively.The%20genetic%20similarity%20coefficient%20among%2024%20cultivars%20ranged from 0.6618 to 0.9485%20,with%20a%20range%20of%200.2867.%20The%20above%20results%20indicate%20that%20the%20genetic%20differences%20among%20the%20tested%20cultivars%20were%20small.TheSdendrogramSof%2024%20cultivars%20wasSconstructedSusingSUPGMA.They%20can%20be%20divided%20into%202%20groups%20at%20genetic%20similarity%20coefficient%20of%200.74,%20of%20which%20there%20were%2022%20cultivars%20in%20the%20first%20group,%20all%20of%20which%20were%20in%20the%20Euro-American%20hybrids%20species;%20there%20were%202%20cultivars%20in%20the%20Group%20II,%20belonging to V.%20vinifera%20×V.%20amurensis%20and%20the%20V.vinifera%20species.%20The%20cultivar%20identification%20diagram%20(CID)%20of%2024%20cultivars%20was%20constructed%20using%20polymorphic%20bands%20by%205%20primers.%20Using%20the%20CID%20,%20we%20can%20find%20the%20primers%20that%20distinguish%20any%20two%20cultivars,%20providing%20scientific%20basis%20for%20the%20identification%20of%20Kyoho%20grapevine%20series. |
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