| 罗立娜.HS-SPME-GC-MS分析油梨种子的挥发性成分[J].中国南方果树,2020,49(4): |
| HS-SPME-GC-MS分析油梨种子的挥发性成分 |
| Analysis of the Volatile Compounds of Avocado seed by HS-SPME-GC-MS |
| 投稿时间：2019-11-27 修订日期：2020-01-21 |
| DOI：10.13938/j.issn.1007-1431.20190698 |
| 中文关键词: 油梨种子, 顶空固相微萃取(HS-SPME), 气相色谱-质谱联用技术(GC-MS), 挥发性成分 |
| 英文关键词:Avocado seed, HS-SPME, GC –MS, Volatile aroma composition |
| 基金项目:贵州省农业科学院项目[黔农科院青年[2018]32号]：油梨种质资源评价、鉴选与利用；贵州省科技厅项目[黔科合支撑[2019]2268号]：油梨良种配套栽培技术集成研究；2019年农业部“滇桂黔石漠化地区油梨品种筛选及配套关键技术集成示范”；2019年农业农村部热作技术试验示范项目“油梨等热带优稀果树新品种选育及技术试验示范”。第一作者：韩树全(1986-),男,硕士,助理研究所,主要从事从事果树育种与栽培。E-mail：651832315@qq.com共同第一作者：罗立娜(1991-),女,硕士,助理研究所,主要从事果树育种与栽培。E-mail：125893926@qq.com HS-SPME-GC-MS分析油梨种子的挥发性成分 |
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| 中文摘要: |
| 本研究采用顶空固相微萃取法提取油梨种子挥发性成分,应用气相色谱-质谱联用技术进行了分离与鉴定,以揭示油梨种子挥发性成分组成。经计算机检索与标准谱图对照,确定其化学成分,并采用峰面积归一法计算各成分的相对含量。从油梨种子中初步鉴定了83 种化学成分,占总挥发性化学成分的86.10%,其中相对含量由高到低依次化合物种类有萜类37种(77.75%)、醇类13种(2.17%)、酯类8种(1.77%)、醛类13种(1.63%)、烃类4种(1.21%)、环氧类2种(0.94%)、酮类3种(0.42%)、肟类1种(0.11%)、酸类1种(0.07%)、噻唑类1种(0.04%),其中主要挥发性化合物有反式-α-佛手柑油烯(27.99%)、大牛儿烯D (11.01%)、反-β-法尼烯(6.85%)、β-倍半水芹烯(4.44%)、反-β-罗勒烯(4.13%)、β-石竹烯(2.82%)、β-甜没药烯(2.56%)、α-蒎烯(2.48%)、δ-杜松烯(2.09%)、反-α-香柠檬烯(1.43%),本研究结果为油梨资源的创新利用提供理论依据。 |
| 英文摘要: |
| Volatile components in the seeds of avocado were studied.The volatile com ponents w ere extracted by headspace solidphase m icroextraction (HS-SPME) and analyzed by gas chrom atography-mass spectrom etry (GC -MS).The relative content of each component was calculated using the peak areas to determine the chemical compositions compared with computer retrieval and standard spectrogram. 83 compounds were identified from avocado seed，which accounted for 86.10% of the volatile chemical components. Comparison of the relative contents of those volatile chemical components showed that: 37 terpenoidss (77.75%), 8 unknown (10.91%), 13 alcohols (2.17%), 8 esters (1.77%), 13 aldehydes (1.63%), 4 hydrocarbons (1.21%), 2 epoxies (0.94%), 3 ketones (0.42%), 1 oximes (0.11%), 1 acid (0.07%), 1 thiazole (0.04%). The main components with higher relative mass fraction were (-)-endo-2,6-dimethyl-6-(4-methyl-3 pentenyl) bicycle[3.1.1]hept-2-ene (27.99%), D-germacrene (11.01%), (E)-β-farnesene(6.85%), β-sesquiphellandrene (4.44%), δ-cadinene (2.09%), (E)-α-bergamotene (1.43%) and etc. The results of this study provide a theoretical basis for the innovative utilization of avocado resources. |
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