| 张沪,肖翠.柑橘TIFY基因的结构特征及响应低温的表达分析[J].中国南方果树,2020,49(2): |
| 柑橘TIFY基因的结构特征及响应低温的表达分析 |
| Structural characteristics of Citrus TIFY gene and its expression in response to low temperature |
| 投稿时间：2019-12-05 修订日期：2020-01-10 |
| DOI： |
| 中文关键词: 柑橘 低温逆境 TIFY 基因结构 表达分析 |
| 英文关键词:Citrus low temperature stress TIFY gene structure expression analysis |
| 基金项目:湖北省农业科学院青年科学基金(2020NKYJJ13)；湖北省自然科学基金面上项目(2019CFB601)；国家重点研发计划(2018YFD1000302)。 |
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| 摘要点击次数: 2036 |
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| 中文摘要: |
| TIFY基因家族是一类包含TIFY结构域的植物特有转录因子,与植物的生长发育密切相关,且在非生物逆境响应中起着重要作用。本研究基于柑橘基因组测序结果,分析了3个TIFY基因家族成员的基因结构特征,并利用qPCR分析了低温胁迫下这些基因的表达水平。结果表明：(1)3个TIFY基因含有TIFY结构域和Jas motif,属于JAZ蛋白亚家族基因；(2)3个TIFY基因的启动子含有LTR、DRE core、STRE、TC-rich repeats等逆境相关顺式作用元件以及MeJA响应元件、ABA响应元件等激素响应相关元件,推测3个TIFY基因可以响应多种非生物胁迫；(3)Cs1g17220和Cs4g07130在果实中高表达,推测其可能与果实发育相关；(4)3个TIFY基因受低温诱导在早期上调表达,且受低温胁迫的程度越大,其受诱导上调表达的倍数越高。本研究将为进一步解析TIFY基因家族在柑橘中的作用和功能提供重要线索。 |
| 英文摘要: |
| The TIFY gene family, a plant-specific family of transcription factors which contain a TIFY domain, play crucial roles in plant developmental processes and in the response to abiotic stress. In this study, gene structure features of three TIFY gene were identified according to the Citrus genome information, and their expression level were analyzed under low temperature stress by qPCR. It was revealed that: (1) The three TIFY gene in this study contain TIFY domain and Jas motif, belonging to the JAZ subfamily. (2) Cis-acting elements involved in stress responsiveness, including LTR, DRE core, STRE and TC-rich repeats, and cis-acting elements involved in plant hormone responsiveness including ABA-responsiveness and MeJA-responsiveness were identified among the promoters of the three TIFY genes, which inferred that the three TIFY genes might respond to multiple abiotic stress. (3) Cs1g17220 and Cs4g07130 showed high expression level in fruit, which inferred that the three TIFY genes might be closely related to fruit development. (4) According to qPCR, the expression of the three TIFY gene were induced at the early stage of low temperature stress and the more serious low temperature stress, the higher level of the gene expression. The results in this study will provide important clues for further elucidating the role and function of TIFY gene family in Citrus. |
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