| 王浩晨.链霉菌30702复合壳聚糖对番木瓜PRSV的防控效果[J].中国南方果树,2020,49(5): |
| 链霉菌30702复合壳聚糖对番木瓜PRSV的防控效果 |
| Control Effect of Streptomyces strain 30702 Composite Chitosan on PRSV of Papaya |
| 投稿时间：2019-12-25 修订日期：2020-03-31 |
| DOI：10.13938/j.issn.1007-1431.20190775 |
| 中文关键词: 木瓜 壳聚糖 链霉菌30702 番木瓜环斑花叶病（PRSV） |
| 英文关键词:Carica papaya L chitosan streptomyces strain 30702 papaya ringspot virus (PRSV) |
| 基金项目:预防番木瓜环斑病毒病的生物农药及其复配增效研究（317009） |
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| 中文摘要: |
| 探究链霉菌30702复合壳聚糖对番木瓜环斑病毒病（papaya ringspot virus，PRSV）的防控效果，为番木瓜环斑花叶病防治提供基础。以台农二号番木瓜为材料，壳聚糖设3个浓度1.0g/L、0.5g/L、0.25g/L；30702菌液2个浓度0 cfu/L和4.2×10 6cfu/L，互相组合为6个处理，以清水处理为空白组，宁南霉素0.05 g/L为对照组，共8个处理；运用分光光度计法测量相关酶活。1.0 g/L壳聚糖复合4.2×106 cfu/L链霉菌30702的防治效果为88.6（p＜0.05），显著高于其他组；壳聚糖复合链霉菌30702组的防治效果、超氧化物歧化酶(SOD)活性、过氧化物酶（POD）活性、多酚氧化酶(PPO)活性相较于单独使用壳聚糖组有显著提升。使用1.0 g/L壳聚糖复合4.2×106 cfu/L的30702对番木瓜防控PRSV的效果最好，防治指数达到88.6%。 |
| 英文摘要: |
| To investigate the control effect of Streptomyces 30702 composite chitosan on papaya ringspot virus (PRSV) disease and provide a basis for the prevention and treatment of papaya ring spot mosaic disease. Using Tainong No. 2 papaya as the material, chitosan was set at three concentrations of 1.0g / L, 0.5g / L, and 0.25g / L; 30702 bacterial solution had two concentrations of 0 cfu / L and 4.2 × 106 cfu / L .Six treatments were combined with water treatment as the blank group and Ningnanmycin 0.05 g / L as the control group. There were 8 treatments in total; the relevant enzyme activities were measured by spectrophotometry. The control effect of 1.0 g / L chitosan 4.2 × 106 cfu / L Streptomyces 30702 was 88.6 (p <0.05), which was significantly higher than that of other groups; The control effect, Superoxide dismutase（SOD）activity, peroxidase（POD）activity, and polyphenol oxidase（PPO）activity of the chitosan-complex streptomyces 30702 group were significantly improved compared with the chitosan group alone. Using 1.0 g / L chitosan combined with 4.2 × 106 cfu / L 30702 had the best effect on controlling PRSV of papaya, and the control index reached 88.6%. |
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