| 马飞跃,张明,帅希祥,杜丽清.神秘果叶粗多糖提取工艺优化及其抗氧化活性研究[J].中国南方果树,2020,49(4): |
| 神秘果叶粗多糖提取工艺优化及其抗氧化活性研究 |
| Optimization of Polysaccharide Extraction from Miracle Fruit Leaves and Antioxidant Activity |
| 投稿时间：2020-04-03 修订日期：2020-05-05 |
| DOI： |
| 中文关键词: 神秘果叶 多糖 抗氧化 |
| 英文关键词:Miracle fruit leaves Polysaccharide Antioxidant activity |
| 基金项目:中央级公益性科研院所基本科研业务费专项(1630062018003)； |
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| 摘要点击次数: 1826 |
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| 中文摘要: |
| 为了更好的开发和利用神秘果资源，优化神秘果叶粗多糖的提取工艺，并评价其抗氧化能力。本研究采用单因素实验对液料比、提取温度、提取时间和提取次数进行研究，在单因素实验基础上，建立三因素三水平的正交试验设计，对提取工艺进行优化，获得最佳提取条件，并测定神秘果叶粗多糖的总抗氧化能力(FRAP)。结果表明提取神秘果叶粗多糖的最佳工艺条件为：液料比25:1 (v/m)、提取温度85 °C、提取时间60 min，提取3次，在此条件下，神秘果叶粗多糖提取率为4.93 ± 0.022%；三个因素对神秘果叶粗多糖提取率影响大小顺序为：提取温度>提取时间>液料比。分析其总抗氧化能力(FRAP)发现，神秘果叶粗多糖具有一定的抗氧化活性(41.38 μmol/g)。 |
| 英文摘要: |
| In order to develop miracle fruit, the extraction process of polysaccharides from miracle fruit leaves was optimized and the antioxidant activity was evaluated. In the present research, single-factor experiments and a three-variable, three-level Orthogonal array design were performed to study on the extraction of polysaccharide from miracle fruit leaves. The optimal conditions were: liquid/solid ratio 25:1 (v/m), extraction temperature 85 °C, extraction time 60 min and 3 extraction cycles. Under the optimal conditions, the average extraction yields of polysaccharide were 4.93 ± 0.022%. The results showed that the impact sequence of the factors on extraction yield of polysaccharide decreased as the follows: extraction temperature >extraction time > liquid/solid ratio. Furthermore, the miracle fruit leaves polysaccharide has antioxidant activity (41.38 μmol/g), according to the total antioxidant capacity assay (FRAP). |
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