| 朱绍志,王芳,孔珊珊,李文清,马电通,李贤忠,邓佳.葡萄柚酵母菌的分离鉴定及对青霉属致病菌的抑菌效果研究[J].中国南方果树,2021,50(2): |
| 葡萄柚酵母菌的分离鉴定及对青霉属致病菌的抑菌效果研究 |
| Isolation and identification of grapefruit yeast and ITS antibacterial effect against Penicillium |
| 投稿时间：2020-04-15 修订日期：2020-12-26 |
| DOI：10.13938/j.issn.1007-1431.20200243 |
| 中文关键词: 酵母菌 葡萄柚 致腐菌 鉴定 抑菌 青霉菌 |
| 英文关键词:Yeast grapefruit saprophytic bacteria identification bacteriostasis Penicillium |
| 基金项目:国家自然科学基金资助项目(31960326)第一作者朱绍志(1991-),男,硕士研究生,研究方向经济林栽培与利用。E-Mail:2260452910@qq.com.通讯作者邓佳(1983-),女,博士。副教授,从事果树培育与利用等研究。E-Mail:dengjia1983@163.com 王芳2 孔珊珊1 李文清1 马电通1 李贤忠1 邓佳3 |
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| 中文摘要: |
| 对葡萄柚的果实、叶片、根际土壤进行酵母菌的分离、鉴定,通过体外、体内抑菌实验,筛选对葡萄柚青霉属致病菌最佳抑菌效果的拮抗酵母菌。通过形态特征和ITS、LSU序列分析,对分离于健康葡萄柚果实、叶片和根际土壤的供试菌株进行鉴定。通过体外抑菌实验,筛选出对青霉属病原菌有较好抑菌效果的拮抗酵母菌,并对其进行体内回接实验,检验其对病原菌的防治效果。结果从样品中分离得到5株酵母菌,其中2株(YY(1)、YY(2))分离于葡萄柚叶片,3株(nf(1)、nf(2)、nf(3))分离于葡萄柚果实,经鉴定分别为：YY(1)(Pseudozyma fusiformata)、YY(2)(Meira nashicola)、nf(1)(Moesziomyces antarcticus)、nf(2)(Meira nashicola)、nf(3)(Kwoniella mangroviensis)。体外抑菌实验表明YY(1)和nf(1)对病原菌JJ-1和YZ-3均有较好的抑菌效果,在第8d时,YY(1)对JJ-1和YZ-3抑菌率分别为55.2%和59%,nf(1)对JJ-1和YZ-3抑菌率分别为16.7%和26.63%。体内实验结果表明,果实接种96h小时,YY(1)对病原菌JJ-1和YZ-3的发病率均为12.5%,病斑抑制率分别为96%和93%；nf(1)对病原菌JJ-1和YZ-3的发病率均为25%,对发病果实病斑抑制率分别为66%和91%。试验表明,拮抗酵母菌YY(1)和nf(1)可以不同程度的降低果实的发病率,抑制果实的病斑直径。拮抗酵母菌YY(1)和nf(1)对病原菌JJ-1、YZ-3的抑菌效果较好。其中,拮抗酵母菌YY(1)对青霉属致腐菌JJ-1、YZ-3的抑菌效果最好,nf(1)次之。 |
| 英文摘要: |
| Through the in vitro and in vivo bacteriostatic experiments, the best Antagonistic Yeast to Penicillium grapefruit was screened. The strains isolated from fruits, leaves and rhizosphere soil of healthy grapefruit were identified by morphological characteristics and ITS, LSU sequence analysis. Through the in vitro bacteriostatic experiment, the Antagonistic Yeast with better bacteriostatic effect on Penicillium was screened out, and ITS in vivo reconnection experiment was carried out to test ITS control effect on pathogenic bacteria. Results five yeasts were isolated from the samples. Two yeasts (YY (1), YY (2)) were isolated from grapefruit leaves, and three yeasts (nf(1), nf(2), nf(3)) were isolated from grapefruit fruits. They were identified as: YY (1) (Pseudozyma fusiformata), YY (2) (Meira nashicola), nf(1) (moesziomyces antarcticus), nf(2) (Meira nashicola), nf(3) (kwoniella mangrovensis). In vitro bacteriostatic experiment showed that YY (1) and nf(1) had good bacteriostatic effect on pathogenic bacteria JJ-1 and YZ-3. On the 8th day, the bacteriostatic rate of YY (1) on JJ-1 and YZ-3 was 55.20% and 59%, and that of nf(1) on JJ-1 and YZ-3 was 16.7% and 26.63%, respectively. The results of in vivo experiments showed that the incidence of YY(1) on JJ-1 and YZ-3 was 12.5%, and the inhibition rate of plaque was 96% and 93%, respectively. The incidence of nf(1) on pathogenic bacteria JJ-1 and YZ-3 was 25%, and the inhibition rate on fruit plaques was 66% and 91%, respectively. The results showed that antagonistic yeasts YY (1) and nf(1) could reduce the incidence of fruit disease and the diameter of fruit spot. The antagonistic yeasts YY (1) and nf(1) had better antibacterial effect on JJ-1 and YZ-3. Among them, the Antagonistic Yeast YY (1) had the best bacteriostatic effect on Penicillium pathogenic bacteria JJ-1 and YZ-3, followed by nf(1). |
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