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刘智成.低温处理对西番莲扦插苗生长及生理特性的影响[J].中国南方果树,2021,50(2):
低温处理对西番莲扦插苗生长及生理特性的影响
Effects of Low Temperature Treatment on the Growth and Physiological Characteristics of Passionfruit Cuttings
投稿时间:2020-06-10  修订日期:2020-08-03
DOI:
中文关键词:  西番莲  扦插苗  低温胁迫  活性酶  生理特性  
英文关键词:Passiflora edulis  Cutting seedling  Low temperature stress  Active enzyme  Physiological characteristics  
基金项目:福建省农业引导性(重点)项目(2017N0049);
作者单位E-mail
刘智成* 福建省龙岩市农业科学研究所 福建龙岩 364000 liuzc510@163.com 
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中文摘要:
      本试验以芭乐黄金果、紫香1号和台农1号西番莲品种扦插苗为试验材料,研究西番莲在低温条件下其植株生长及其体内保护酶活性变化情况,并通过恢复生长试验,测定植株存活率,进一步探明西番莲耐冷机理。试验结果表明,在低温处理下,各参试材料中丙二醛(MDA)和可溶性糖(SS)含量随温度降低而逐渐增加;超氧化物歧化酶(SOD)活性测得值呈下降趋势、过氧化物酶(POD)活性、过氧化氢酶(CAT)活性和脯氨酸(Pro)含量测得值呈先增加后减少,在温度为10℃时,测得值均达到最大,在温度低于3℃时,测得值均低于对照;恢复生长试验中,3℃处理后的芭乐黄金果西番莲品种存活率为0,0℃处理后的三个参试品种,存活率均为0。综上所述,西番莲扦插苗受到低温伤害时,其叶片中MDA含量增加,诱导出POD、SOD和CAT保护酶活性,同时体内脯氨酸(Pro)和可溶性糖(SS)含量会逐渐积累增加,从而进一步提高植株抗寒性。
英文摘要:
      The test used the cuttings of Balehuangjinguo, Zixiang NO. 1 and Tainong NO.1 as experimental materials, In order to study the plant growth of Passiflora edulis and the change of protective enzyme activity in the at low temperatureplant, and the survival rate of the plants was determined by recovery growth test, thus further explore the cold-resistant mechanism of Passiflora edulis. The results showed that the content of malondialdehyde (MDA) and soluble sugar (SS) increased gradually, the activity of superoxide dismutase (SOD) decreased gradually, the measured values of oxidase (POD) activity, catalase (CAT) activity and proline (Pro) content increased first and then decreased with the decrease of temperature. When the temperature was 10℃, the measured values all reached the maximum. When the temperature was below 3℃, the measured values were all lower than the control. In the recovery growth test, the survival rate of Balehuangjinguo varieties was 0 under the 3℃ treatment, and the three test varieties after 0℃ treatment was 0. In summary, when cutting seedlings of Passiflora edulis were harmed by low temperature, the MDA content in their leaves increased, which induced activities of POD, SOD and CAT protective enzymes, and meanwhile, contents of proline (Pro) and soluble sugar (SS) in vivo gradually increased, thus further improving the cold resistance of plants.
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