| 朱 丽,熊岑,刘苗,周思.山西贡梨粗多糖的提取工艺优化及其抗氧化活性研究[J].中国南方果树,2021,50(4): |
| 山西贡梨粗多糖的提取工艺优化及其抗氧化活性研究 |
| Study on the Optimization of Extraction Technology and Antioxidant Activity Identification of the Crude Polysaccharide in Shanxi Gong Pear |
| 投稿时间：2020-08-11 修订日期：2021-04-21 |
| DOI： |
| 中文关键词: 山西贡梨 粗多糖 正交试验 抗氧化活性 |
| 英文关键词:Shanxi Gong pear Crude polysaccharides Orthogonal test Antioxidant activity |
| 基金项目: |
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| 摘要点击次数: 1737 |
| 全文下载次数: 334 |
| 中文摘要: |
| 选取山西贡梨为研究对象，使用超声辅助的方法提取山西贡梨粗多糖，采用单因试验探讨了提 取 温 度、提取时间、液料比和提取功率的影响；在 单 因 素 试 验 基 础 上，再进行正交试验优化最佳的工艺提取条件。根据正交试验结果分析得最佳的提取条件为：提取温度为60 ℃、提取20 min、料液比配1:20（g/mL）、超声功率120 W。采用最佳的提取工艺条件对山西贡梨粗多糖进行提取，提取率可达15.43%。以抗坏血酸作为阳性对照，测试山西贡梨粗多糖对1,1-二苯基-2-苦肼基（DPPH?）和羟基自由基（?OH）的清除率，试验结果显示山西贡梨粗多糖与抗坏血酸对上述两种自由基均表现出较明显的清除能力，且清除能力与粗多糖浓度正相关性。山西贡梨粗多糖的抗氧化性研究为山西贡梨精加工产品研提供一定的前期理论基础。 |
| 英文摘要: |
| In this study, the raw material of the fresh the crude polysaccharide of Shanxi Gong pear was extracted by ultrasonic auxiliary method,. aBased on single factor experiments，The effects of extraction temperature, extraction time, liquid-solid ratio and extraction power were studied,nd the influence of the four extraction factors on the crude polysaccharide extraction rate of Gong pear was explored by the ratio of material liquid, ultrasonic power, the temperature and time of extraction. The optimum extraction conditions were optimized by orthogonal test.First, the optimal single-factor level is selected by single-factor test, and then the optimal process extraction conditions are selected by orthogonal test. According to the results of orthogonal test, the best extraction conditions are as follows: ultrasonic power was 120 W, extraction temperature was 60℃， extraction time was 20 min,material ratio was 1:20 (g/mL), ultrasonic power was 120 W,extraction temperature and time were 60℃ and 20 min, respectively. The extraction rate of the crude polysaccharide of Shanxi Gong pear was carried out with the best extraction process condition, and the extraction rate was up to 15.43% with the optimal condition. With ascorbic acid as a positive control, the positive correlation between gonryl coarse polysaccharide and 1,1-diphben-2-bitter-free (DPPH?) and hydroxyl free radicals (?OH) was carried out, and the results showed that the polysaccharide and ascorbic acid of gin showed a more obvious ability to remove the above two free radicals, and the removal capacity was positively correlated with the concentration of crude polysaccharide. The study on antioxidant activity of Shanxi Gongli crude polysaccharide can provide a certain theoretical basis for the research of Shanxi Gongli refined processing products. The study on antioxidation of Gong pear crude polysaccharide provided a theoretical basis for the study on the components of Gong pear polysaccharide and the development of refined products. |
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