| 屈艺,曾明.丛枝菌根真菌和糖醇螯合钙对草莓采后果实硬度和细胞壁酶活及其关键基因表达的影响[J].中国南方果树,2021,50(4): |
| 丛枝菌根真菌和糖醇螯合钙对草莓采后果实硬度和细胞壁酶活及其关键基因表达的影响 |
| Effects of arbuscular mycorrhizal fungi and sugar alcohol chelated calcium on firmness, cell wall enzyme activities and key gene expression of postharvest strawberry fruits |
| 投稿时间：2020-12-29 修订日期：2021-02-23 |
| DOI：10.13938/j.issn.1007-1431.20200869 |
| 中文关键词: 果实 硬度 丛枝菌根真菌 糖醇螯合钙 细胞壁酶活性 基因表达 |
| 英文关键词:Fruit Firmness Arbuscular mycorrhizal fungi Sugar alcohol chelated calcium Cell wall enzyme activity Gene expression |
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| 中文摘要: |
| 以盆栽‘红颜’草莓为试材，研究了摩西管柄囊霉（Funneliformis mosseae）和糖醇螯合钙不同浓度（0.10%、0.15%、0.20%）处理对草莓采后果实硬度和细胞壁酶活的变化，同时，初步探究了不同处理对草莓果实软化关键基因FaPG1、FaβGal4的表达水平的影响。结果表明，与对照相比，以糖醇螯合钙单独施用（0.15%和0.20%）、接种丛枝菌根真菌（Arbuscular mycorrhizal fungi, AMF）配施糖醇螯合钙均能显著提高草莓果实硬度。同时，不同处理均抑制多聚半乳糖醛酸酶（PG）、果胶甲酯酶（PME）、β-半乳糖苷酶（β-Gal）活性的上升，草莓果实软化关键基因FaPG1和FaβGal4在处理后均下调表达。在接种AMF条件下，随着糖醇螯合钙浓度的增加，果实硬度逐渐增强，其中以接种AMF联合施用0.20%糖醇螯合钙溶液效果最好。 |
| 英文摘要: |
| Potted ''Hongyan'' strawberry was used to study the changes of firmness and cell wall enzyme activities of strawberry fruits treated with Funneliformis mosseae and different concentrations of sugar alcohol chelated calcium. Meanwhile, The effects of different treatments on the expression levels of key genes FaPG1 and FaβGal4 in strawberry fruit softening were studied. The results showed that compared with the control, the firmness of strawberry fruit could be significantly improved by applying sugar alcohol chelated calcium alone or inoculating arbuscular mycorrhizal fungi with sugar alcohol chelated calcium. At the same time, the activities of polygalacturonase (PG), pectin methylesterase (PME) and β-galactosidase (β-gal) were inhibited by different treatments, and the expression of key genes FaPG1 and FaβGal4 were down regulated after treatment. Under the condition of AMF inoculation, fruit firmness increased with the increase of sugar alcohol chelated calcium concentration, and the best effect was AMF inoculation combined with 0.20% sugar alcohol chelated calcium solution. |
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