| 邹毅辉,徐婧君,陈育青,黄红宣.连作对百香果根际土壤微生物群落的影响[J].中国南方果树,2022,51(1): |
| 连作对百香果根际土壤微生物群落的影响 |
| Effects of continuous cropping on microbial community in rhizosphere soil of Passiflora edulis Sims |
| 投稿时间：2021-02-06 修订日期：2021-04-20 |
| DOI： |
| 中文关键词: 百香果 连作障碍 高通量测序 土壤微生物 微生物群落结构 |
| 英文关键词:Passiflora edulis Sims Continuous cropping obstacle high-throughput sequencing technology soil microorganism microbial community structure |
| 基金项目:2019年福建省科技特派员资助项目(闽科农[2019]11号)，漳州卫生职业学院院级科研项目（ZWYZ201909） |
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| 中文摘要: |
| 摘 要：以连作1年和连作3年的百香果根际土壤作为研究对象，采用Illumina MiSeq高通量测序技术分析根际土壤中的微生物群落结构组成和多样性变化，以期为克服百香果连作障碍提供指导。结果表明：连作显著降低百香果根际土壤细菌群落多样性和真菌群落丰富度，增加百香果根际土壤的细菌群落丰富度和真菌群落多样性。根际土壤微生物群落组成发生了较大的变化，在门分类水平上，连作三年的根际土壤中变形菌门（Proteobacteria）和厚壁菌门（Firmicutes）相对丰度增加了5.86%和2.21%；放线菌门（Actinobacteria）减少了3.40%，子囊菌门（Ascomycota）减少了28.41%，而毛霉门（Mucoromycota）增加了14.73%，在属分类水平上，连作三年的根际土壤中根际土壤中的γ-变形菌纲中未分类属unclassified_Gammaproteobacteria、黄单胞杆菌科未分类属unclassified_Xanthomonadaceae相对丰度显著增加，酸杆菌门中而Gp2相对丰度显著降低，子囊菌属（Archaeorhizomyces）、孢子丝菌属（Sporothrix）、粪壳菌属（Sordaria）相对丰度减少，小被孢霉（Mortierella）、镰刀菌属（Fusarium）两个病原菌属相对丰度增加。结论：连作改变了百香果根际土壤微生物群落结构，细菌多样性降低，真菌多样性升高，病原菌相对丰度增加，土壤由细菌型向真菌型转化。 |
| 英文摘要: |
| Abstract: In order to provide guidance for overcoming the obstacles of continuous cropping of passion fruit, the microbial community structure composition and diversity in the rhizosphere soil of passion fruit were analyzed by IIIumina Miseq high-throughput sequencing technology with 1-year and 3-year continuous cropping as the research object. The results showed that continuous cropping significantly decreased the bacterial community diversity and fungal community richness in the rhizosphere soil of passionfruit, while increased the bacterial community richness and fungal community diversity in the rhizosphere soil. The microbial community composition of rhizosphere soil changed greatly. At the taxonomic level, the relative abundance of Proteobacteria and Firmicutes in rhizosphere soil increased by 5.86% and 2.21% after three years of continuous cropping. Actinobacteria decreased by 3.40%, Ascomycota decreased by 28.41%, and Mucoromycota increased by 14.73%. At the level of genus classification, the relative abundance of γ-proteobacteria and Unclassified_gammaproteobacteria and Unclassified_xanthomonadaceae in the rhizosphere soil after three years of continuous cultivation significantly increased. In Acidobacteria, the relative abundance of GP2 decreased significantly, the relative abundance of Archaeorhizomyces, Sporothrix and Sordaria decreased, and the relative abundance of Mortierella and Fusarium increased. Conclusion: Continuous cropping changed the microbial community structure of rhizosphere soil of passion fruit, the bacterial diversity decreased, the fungal diversity increased, the relative abundance of pathogenic bacteria increased, and the soil transformed from bacterial type to fungal type. |
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