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刘敏竹,李强,杨超,韩涛,凌丽俐,付行政,曹立,何义仲.不同LED光质对红桔幼苗生长发育和叶绿素荧光特性的影响[J].中国南方果树,2021,50(2):
不同LED光质对红桔幼苗生长发育和叶绿素荧光特性的影响
Effects of Different LED Light Quality on Growth, Development and Chlorophyll Fluorescence Characteristics of Citrus tangerine Seedlings
投稿时间:2021-02-16  修订日期:2021-02-16
DOI:
中文关键词:  红桔  LED  光质  生长发育  叶绿素荧光特性
英文关键词:tangerine  LED  light quality  growth and development  chlorophyll fluorescence characteristics
基金项目:国家现代农业(柑橘)产业技术体系建设专项(CARS-26-01A);重庆市技术创新与应用发展专项(cstc2019jscx-gksbX0102);中央高校基本业务费专项资金资助(XDJK2019B041)
作者单位E-mail
刘敏竹 西南大学柑桔研究所 736214409@qq.com 
李强 重庆文理学院化学与环境工程学院 lq1973_2002@163.com 
杨超 西南大学柑桔研究所 1005309602@qq.com 
韩涛 重庆文理学院化学与环境工程学院 danbaiht@126.com 
凌丽俐* 西南大学柑桔研究所 linglili@cric.cn 
付行政 西南大学柑桔研究所 fuxingzheng@cric.cn 
曹立 西南大学柑桔研究所 caoli@cric.cn 
何义仲 西南大学柑桔研究所 heyzh2018@swu.edu.cn 
摘要点击次数: 1927
全文下载次数: 2341
中文摘要:
      为了阐明不同LED光质及配比对红桔幼苗生长和叶绿素荧光特性的影响,以水培红桔幼苗为试验材料,LED白光(W)为对照,设置LED红光(R)、蓝光(B)、红蓝复合光(RB11、RB41)、红绿蓝复合光(RGB411)等5个处理。统计、测定幼苗的表型、生物量、光合色素含量和叶绿素荧光特征参数,并对相关指标进行主成分分析。结果表明:与对照相较,红蓝单色光和复合光显著抑制红桔幼苗根茎叶的生长,而在高比例红蓝复合光中添加绿光(RGB411)能显著促进幼苗的根伸长、茎增高、茎增粗和植株叶面积扩增,其主根长、株高、茎粗和株叶面积分别比对照大4.0%(P>0.05)、18.8%(P<0.05)、11.1%(P>0.05)和28.7%(P<0.05),更有利于地上部分(茎、叶)的形态建成和光吸收;红蓝单色光和复合光均导致幼苗根茎叶的生物量呈降低趋势,仅添加绿光的RGB411组在株鲜质量、株干质量、叶干质量和地上部分干质量上均呈增长趋势,分别比对照重4.0%(P>0.05)、18.8%(P<0.05)、11.1%(P>0.05)和28.7%(P<0.05);在红蓝复合光中仅RB11组的光合色素含量呈增加趋势,其chla、chlb、chl和car的含量分别比对照高5.3%(P>0.05)、8.1%(P>0.05)、5.8%(P>0.05)和7.2%(P>0.05),而添加绿光的RGB411组的叶绿素含量呈降低趋势;红光导致幼苗的光合作用能力显著降低,蓝光则呈相反趋势,而红蓝复合光和红绿蓝复合光的光合作用能力处于红、蓝单色光之间。经主成分分析,LED红绿蓝复合光(RGB411)更有利于红桔幼苗的生长发育和物质积累,为建立优质红桔类苗木快速繁育的LED补光技术提供了理论和实验依据。
英文摘要:
      In order to clarify the effects of different LED light qualities and ratios on the growth and chlorophyll fluorescence characteristics of tangerine seedlings, tangerine seedlings cultivated with nutrient solution were selected as test materials. The seedlings were raised in a culture room with a temperature of 25±2℃, and the photoperiod was set to 12/12h, and the light intensity was adjusted to 150μmol·m-1·s-1. With white light as a control, the seedlings grew under LED red light (R),blue light (B) ,red-blue composite light (RB11, RB41), and red-green-blue composite light (RGB411), respectively. The phenotypic index, biomass accumulation, photosynthetic pigment content and chlorophyll fluorescence characteristic parameters of the seedlings were measured, and the principal component analysis was performed on these indicators.The results showed that red and blue light and compound light significantly inhibited the growth of the roots, stems and leaves of tangerine seedlings compared with the control, while Seedlings exposed to RGB411 light had longer roots, taller and thicker stems, and larger leaves. Its main root length, plant height, stem thickness and plant leaf area were 4.0% (P>0.05), 18.8% (P<0.05), 11.1% (P> 0.05) and 28.7% (P<0.05)larger than the control, respectively. It is more conducive to the morphology and light absorption of the above-ground parts (stems and leaves). Both red, blue light and compound light cause the biomass of seedling roots, stems and leaves to decrease, only the RGB411 group showed an increasing trend in plant fresh quality, plant dry quality, leaf dry quality and aerial dry quality, which were 4.0% (P>0.05), 18.8% (P<0.05), 11.1% ( P>0.05) and 28.7% (P<0.05) heavier than the control. In the red-blue combined light, only the photosynthetic pigment content of the RB11 group increased, and the content of chla, chlb, chl and car were respectively higher than the control by 5.3% (P>0.05 ), 8.1% (P>0.05), 5.8% (P>0.05) and 7.2% (P>0.05), while the chlorophyll content of the RGB411 group decreased. Red light significantly reduces the photosynthesis capacity of seedlings, while blue light has the opposite effect, while the photosynthesis capacity of red-blue composite light and red-green-blue composite light is between red and blue light. Through principal component analysis, it can be found that LED red-green-blue composite light (RGB411) is more conducive to the growth and material accumulation of tangerine seedlings, which provides theoretical and experimental basis for the establishment of LED supplement light technology for rapid propagation of high-quality tangerine seedlings.
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