| 张惠茜,谢江辉.香蕉枯萎病拮抗放线菌的分离鉴定及抑菌机理分析[J].中国南方果树,2021,50(3): |
| 香蕉枯萎病拮抗放线菌的分离鉴定及抑菌机理分析 |
| Isolation, Identification and Antagonistic Mechanism of Actinomycetes JRGG-11 Against Banana Fusarium Wilt |
| 投稿时间：2021-03-14 修订日期：2021-04-19 |
| DOI： |
| 中文关键词: 香蕉枯萎病 放线菌 分离鉴定 防控效果 抑菌机理 |
| 英文关键词:Banana Fusarium wilt actinomycetes isolation and identification control effect inhibiting mechanism. |
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| 中文摘要: |
| 为丰富香蕉枯萎病生防菌资源，探究其抑菌机理，从润楠树（Machilus pingii ）根系土壤中分离筛选对香蕉枯萎病具有拮抗作用的放线菌，基于16S rRNA序列进化分析和生理生化特征，鉴定其种属。利用激光共聚焦显微镜评测盆栽试验的防控效果，并通过扫描电镜、透射电镜分析其对孢子形态结构及超微结构的影响。筛选出一株拮抗效果较好的放线菌，鉴定为链霉菌属，命名为JRGG-11，该菌对枯萎病四号生理小种（Foc 4）的抑菌率为80.48±1. 49%，发酵液的抑菌率为58.77±1.31%。盆栽试验显示JRGG-11发酵液对Foc 4的防控指数为83.1%。菌株JRGG-11提取物对Foc 4菌丝生长抑制的EC50为18.70 μg/ml。当用4×EC50处理时，Foc 4菌丝形态和细胞完整性被严重破坏，孢子萌发完全被抑制。结果表明，链霉菌JRGG-11具有较好的生防潜能。 |
| 英文摘要: |
| To enrich the resources of banana fusarium wilt biocontrol bacteria and explore the antibacterial mechanism, actinomycetes with antagonistic effects on banana fusarium wilt were isolated and screened from the root soil of Machilus pingii. The candidate strain was identified by 16S rRNA sequence analysis and physiological and biochemical characteristics. The inhibitory effect was comprehensively evaluated according to the results of pot experiments by a confocal laser scanning microscope. The effects of actinomycetes on the morphology and ultrastructure of spores were investigated by a scanning electron microscopy and a transmission electron microscopy. A strain marked with JRGG-11 showed a high antagonistic effect on Foc TR4. The inhibition rates of strain JRGG-11 and its extracts were 80.48±1.49% and 58.77±1.31%, respectively. Strain JRGG-11 was identified as Streptomyces sp.．The pot experiment showed that the fermentation broth can obviously inhibit the infection of Foc 4，the inhibition rate was 83.1%. EC50 value of the strain extracts against Foc 4 was 18.70 μg/mL. The morphology and cell integrity of mycelia were seriously destroyed and the conidia germination was completely inhibited under 4×EC50 of extract treatment. The results showed that Streptomyces sp.JRGG-11 has a well potential for biological control. |
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