| 胡亚平,郭雁君,吉前华,杨凤梅,蒋惠,郭丽英.柑橘几丁质酶基因家族的结构、分类与进化分析[J].中国南方果树,2022,51(6): |
| 柑橘几丁质酶基因家族的结构、分类与进化分析 |
| Structure, classification and evolution of chitinase gene family in Citrus |
| 投稿时间：2021-05-04 修订日期：2021-05-25 |
| DOI：10.13938/j.issn.1007-1431.20210291 |
| 中文关键词: 柑橘 几丁质酶 基因家族 |
| 英文关键词:Citrus chitinase gene family |
| 基金项目:广东省自然科学基金项目，沙糖桔几丁质酶基因家族的抗病功能研究 |
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| 中文摘要: |
| 【目的】植物的几丁质酶与其抗病能力、防御反应和生长发育密切相关，本文通过生物信息学方法来研究柑橘几丁质酶基因家族成员的性质。【方法】通过检索柑橘全基因组序列，发现了32个几丁质酶基因，使用生物信息学软件对这些基因进行分子量、等电点、信号肽、细胞定位、转录谱、保守结构域、系统发育树等方面进行研究。【结果】该家族基因中有26个成员定位在染色体上，其余6个位置尚未确定。这些几丁质酶基因家族成员的蛋白分子量、氨基酸残基和等电点分别位于14.8～72.7KDa、134～636个和4.53～9.21之间。。除3个预测蛋白不含信号肽外，其余均含有信号肽，其中23个蛋白定位在细胞外，6个蛋白横跨细胞膜。对这些基因在花、叶、果实和愈伤等组织的转录组测序数据进行分析，发现它们的转录水平相差巨大，有的在几种组织中转录水平都很高，有的在不同组织中转录水平相差很大，有的在所有组织中表达量都很低。用最大似然法对蛋白序列构建系统发育树，结合保守结构域的分析结果，可将这些几丁质酶基因分为4类。第1类有信号肽且定位在细胞外，有GH18几丁质酶结构域，同时具有糖基水解酶的功能；第2类大部分是带信号肽的跨膜蛋白，也有GH18几丁质酶结构域，还有一个丝氨酸/苏氨酸激酶的结构域；第3类带信号肽且定位在细胞外，有GH19几丁质酶结构域，同时具有溶菌酶的活性；第4类仅有一个基因，是个无信号肽的跨膜蛋白，有GH18几丁质酶结构域，同时具有糖基水解酶的功能，除此之外还含有抗病蛋白中常见的PPR重复结构域。【结论】对柑橘几丁质酶家族进行分析，有助于了解几丁质酶抗病的分子机制，找出起主效抗病作用的几丁质酶基因，从而为提高柑橘的抗病育种提供帮助。 |
| 英文摘要: |
| 【Objective】plant chitinase is closely related to its disease resistance, defense response and growth and development. In this paper, the properties of chitinase gene family members in citrus were studied by bioinformatics methods.【Methods】32 chitinase genes were found by searching the whole genome sequence of citrus. The molecular weight, isoelectric point, signal peptide, cell localization, transcriptional profile, conserved domain and phylogenetic tree of these genes were studied by using bioinformatics software.【Results】26 members of the family were located on chromosomes, and the other 6 were not identified. The molecular weight, amino acid residues and isoelectric point of these chitinase gene family members were 14.8-72.7kda, 134-636 and 4.53-9.21, respectively. Except for 3 predicted proteins, all of them contained signal peptides. Among them, 23 proteins were located outside the cell and 6 proteins were located across the cell membrane. By analyzing the transcriptome sequencing data of these genes in flowers, leaves, fruits and calli, it was found that the transcriptional levels of these genes vary greatly. Some of them have high transcriptional levels in all tissues, some have high transcriptional levels in different tissues, and some have low expression levels in all tissues. These chitinase genes can be divided into four groups according to the analysis of conserved domains. The first group has signal peptide and is located outside the cell. It has gh18 chitinase domain and glycosylhydrolase function; Most of the second group are transmembrane proteins with signal peptide, including gh18 chitinase domain and serine / threonine kinase domain; The third group has signal peptide and is located outside the cell. It has gh19 chitinase domain and lysozyme activity; There is only one gene in group 4, which is a transmembrane protein without signal peptide. It has gh18 chitinase domain and glycohydrolase function. In addition, it also contains PPR repeat domain which is common in disease resistance proteins【Conclusion】 the analysis of chitinase family in citrus is helpful to understand the molecular mechanism of chitinase resistance and find out the chitinase genes that play a major role in disease resistance, so as to provide help for improving citrus disease resistance breeding. |
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